التفاصيل البيبلوغرافية
| العنوان: |
Trials to define biosensor control of MRSA proliferation in vivo . |
| المؤلفون: |
Changhui Guan (6261452), Peter J. Larson (14265589), Elizabeth Fleming (217050), Alexander P. Tikhonov (14265592), Sara Mootien (431957), Trudy H. Grossman (1585339), Caroline Golino (14265595), Julia Oh (240419) |
| سنة النشر: |
2022 |
| مصطلحات موضوعية: |
Medicine, Microbiology, Biotechnology, Immunology, Developmental Biology, Cancer, Infectious Diseases, Virology, Space Science, Environmental Sciences not elsewhere classified, Biological Sciences not elsewhere classified, Mathematical Sciences not elsewhere classified, significant clinical concern, selectively deliver antimicrobials, numerous beneficial functions, engineered skin probiotic, div >< p, prolonged antibiotic exposure, >) aureus <, staphylococcus aureus <, aureus <, antibiotic resistance, vitro <, epidermidis <, “ detect, producing lysostaphin, pathogen colonization, mrsa ), discuss challenges |
| الوصف: |
Germ-free C57BL6/J mice were colonized on the ears on days 0, 2 and 4, and growth of S . epidermidis ( agr type I lysostaphin producing biosensor or Tü3298Δ agr parent strain) and S . aureus was quantified by swabbing the ear, directly spreading on mannitol salt agar, and then counting CFUs on days 2, 4, 6, 8, and 10 (select cases). In A) we show representative control samples for colonization alone by S . aureus USA300 (MRSA), S . epidermidis parent, or S . epidermidis biosensor strains. B) and C) , D) and E) , and F) and G) are replicate experiments. In B) and C) , we examined biosensor efficacy in suppressing growth of MRSA when co-colonized. MRSA was mixed with the biosensor or parent in a suspension at a 1:100 ratio (10 7 /10 9 CFUs, respectively) and then applied to the mouse ears. n = 5 mice for all groups. Spearman linear regression line is shown with 95% confidence intervals. P-value to assess statistical significance in CFU counts between groups, accounting for time as a covariate, was determined within each panel by ANCOVA. In D) and E) , we examined biosensor efficacy in preventing colonization (and suppressing growth), colonizing mouse ears with biosensor or parent alone at day 0, then challenging with the suspension of S . aureus USA300 with S . epidermidis biosensor or parent on day 2. n = 5 for all groups. In F) and G) , we examined growth in a wound model, in which mice underwent a dorsal punch biopsy which was then inoculated with the suspension of S . aureus USA300 plus biosensor or parent. After 48 h, dorsal skin surrounding the wound was harvested for CFU quantitation on mannitol salt agar. n = 5 mice/group (left), n = 12/group (right). P-value was determined by bidirectional t-test. |
| نوع الوثيقة: |
still image |
| اللغة: |
unknown |
| Relation: |
https://figshare.com/articles/figure/Trials_to_define_biosensor_control_of_MRSA_proliferation_i_in_vivo_i_/21733864 |
| DOI: |
10.1371/journal.pone.0276795.g003 |
| الاتاحة: |
https://doi.org/10.1371/journal.pone.0276795.g003 |
| Rights: |
CC BY 4.0 |
| رقم الانضمام: |
edsbas.1047FE10 |
| قاعدة البيانات: |
BASE |