التفاصيل البيبلوغرافية
| العنوان: |
Additional file 8 of Novel γ-sarcoglycan interactors in murine muscle membranes |
| المؤلفون: |
Tara C. Smith (8843486), Georgios Vasilakos (11989234), Scott A. Shaffer (153908), Jason M. Puglise (11989237), Chih-Hsuan Chou (3135186), Elisabeth R. Barton (11989240), Elizabeth J. Luna (11989243) |
| سنة النشر: |
2022 |
| المجموعة: |
Smithsonian Institution: Digital Repository |
| مصطلحات موضوعية: |
Biophysics, Biochemistry, Medicine, Cell Biology, Genetics, Molecular Biology, Physiology, Infectious Diseases, Limb girdle muscular dystrophy, Skeletal muscle, Sarcolemma, Archvillin, Svil, PP1β/δ, Sarcoglycans, NKCC1 |
| الوصف: |
Additional file 8: Figure S5. Validation of rabbit (Rb) polyclonal antibodies against NKCC1. (A, C) Full-length immunoblots of mouse muscle and RH30 cells with and without transfected HA-CFP-NKCC1; (B, D) Anti-NKCC1 (panels a, d; red in merges) and murine (M) anti-HA staining (panels b, e; blue in merges) in RH30 cells transfected with HA-CFP-NKCC1. Anti-NKCC1 antibodies were from (A, B) Alomone Labs, #ANT-071 and (C, D) Proteintech Group, #13884-1-AP. Both anti-NKCC1 antibodies recognized the expected bands in the 97-220 kDa molecular mass range (A, C). Similar staining patterns have been reported previously, with the multiplicity of bands attributed to differential splicing and variations in glycosylation [74, 75]. In immunofluorescence (B, D), only the Proteintech antibody (Da, Dd) exhibited enhanced staining in RH30 cells expressing high levels of exogenous HA-CFP-NKCC1 (B, D, arrows). Overlapping signals appear as magenta in Merges (c and f). Bars, 20 μm. |
| نوع الوثيقة: |
still image |
| اللغة: |
unknown |
| Relation: |
https://figshare.com/articles/figure/Additional_file_8_of_Novel_-sarcoglycan_interactors_in_murine_muscle_membranes/18866563 |
| DOI: |
10.6084/m9.figshare.18866563.v1 |
| الاتاحة: |
https://doi.org/10.6084/m9.figshare.18866563.v1 |
| Rights: |
CC BY + CC0 |
| رقم الانضمام: |
edsbas.101FD0E7 |
| قاعدة البيانات: |
BASE |