Academic Journal
Comparison of extraction procedures and determination of the detection threshold for Clavibacter michiganensis ssp. michiganensis in tomato seeds
| العنوان: | Comparison of extraction procedures and determination of the detection threshold for Clavibacter michiganensis ssp. michiganensis in tomato seeds |
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| المؤلفون: | Hadas, R., Kritzman, G., Klietman, F., Gefen, T., Manulis, S. |
| المصدر: | Plant Pathology ; volume 54, issue 5, page 643-649 ; ISSN 0032-0862 1365-3059 |
| بيانات النشر: | Wiley |
| سنة النشر: | 2005 |
| المجموعة: | Wiley Online Library (Open Access Articles via Crossref) |
| الوصف: | Several seed extraction procedures, used for detection of Clavibacter michiganensis ssp. michiganensis ( Cmm ) in naturally infected and artificially infested tomato seed lots were evaluated. Extraction methods that included grinding the seeds were significantly better at detecting the pathogen in three different seed lots than methods that used only soaking. The detection threshold of Cmm in relation to seed sample size was determined by adding naturally infected seeds into samples of three different sizes. Cmm was detected by agar plating assay, on three media (CNS, mSCM, D 2 ANX), and by direct PCR from seeds and Bio‐PCR (bacteria cultured on agar media prior to PCR). In samples of 10 000 seeds containing one infected seed, Cmm could be detected only by Bio‐PCR and in only one replicate out of five. In samples containing five or 10 infected seeds per 10 000 seeds, three of five and five of five replicates, respectively, were detected by the three detection methods. In samples of 5000 seeds, one infected seed could be detected in all five replicates only after adding a concentration step. A high correlation ( R 2 = 0·9448) between artificially infested seeds and the disease incidence was found. Seed lots infested with less than 58 colony‐forming units (CFU) per g did not cause disease under glasshouse conditions, whereas lots with about 1000 CFU g −1 caused disease in 78 plants out of 2000. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1111/j.1365-3059.2005.01230.x |
| DOI: | 10.1111/j.1365-3059.2005.01230.x/fullpdf |
| الاتاحة: | http://dx.doi.org/10.1111/j.1365-3059.2005.01230.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1365-3059.2005.01230.x http://onlinelibrary.wiley.com/wol1/doi/10.1111/j.1365-3059.2005.01230.x/fullpdf |
| Rights: | http://onlinelibrary.wiley.com/termsAndConditions#vor |
| رقم الانضمام: | edsbas.101383BE |
| قاعدة البيانات: | BASE |
| DOI: | 10.1111/j.1365-3059.2005.01230.x |
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