التفاصيل البيبلوغرافية
| العنوان: |
Single-shot refractive index slice imaging using spectrally multiplexed optical transfer function reshaping |
| المؤلفون: |
Lee, Chungha, Hugonnet, Herve, Park, Juyeon, Lee, Mahn Jae, Park, Weisun, Park, Yongkeun |
| سنة النشر: |
2023 |
| المجموعة: |
Physics (Other) |
| مصطلحات موضوعية: |
Physics - Optics, Electrical Engineering and Systems Science - Image and Video Processing, Physics - Biological Physics |
| الوصف: |
The refractive index (RI) of cells and tissues is crucial in pathophysiology as a noninvasive and quantitative imaging contrast. Although its measurements have been demonstrated using three-dimensional quantitative phase imaging methods, these methods often require bulky interferometric setups or multiple measurements, which limits the measurement sensitivity and speed. Here, we present a single-shot RI imaging method that visualizes the RI of the in-focus region of a sample. By exploiting spectral multiplexing and optical transfer function engineering, three color-coded intensity images of a sample with three optimized illuminations were simultaneously obtained in a single-shot measurement. The measured intensity images were then deconvoluted to obtain the RI image of the in-focus slice of the sample. As a proof of concept, a setup was built using Fresnel lenses and a liquid-crystal display. For validation purposes, we measured microspheres of known RI and cross-validated the results with simulated results. Various static and highly dynamic biological cells were imaged to demonstrate that the proposed method can conduct single-shot RI slice imaging of biological samples with subcellular resolution. |
| نوع الوثيقة: |
Working Paper |
| DOI: |
10.1364/OE.485559 |
| URL الوصول: |
http://arxiv.org/abs/2301.05425 |
| رقم الانضمام: |
edsarx.2301.05425 |
| قاعدة البيانات: |
arXiv |