Report
Nanoscale topography and poroelastic properties of model tissue breast gland basement membranes
| العنوان: | Nanoscale topography and poroelastic properties of model tissue breast gland basement membranes |
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| المؤلفون: | Fabris, Gloria, Lucantonio, Alessandro, Hampe, Nico, Noetzel, Erik, Hoffmann, Bernd, DeSimone, Antonio, Merkel, Rudolf |
| المصدر: | Biophysical Journal 115, 1770-1782, 2018 |
| سنة النشر: | 2018 |
| المجموعة: | Condensed Matter Physics (Other) Quantitative Biology |
| مصطلحات موضوعية: | Physics - Biological Physics, Condensed Matter - Soft Condensed Matter, Quantitative Biology - Tissues and Organs |
| الوصف: | Basement membranes (BMs) are thin layers of condensed extracellular matrix proteins serving as permeability filters, cellular anchoring sites, and barriers against cancer cell invasion. It is believed that their biomechanical properties play a crucial role in determining cellular behavior and response, especially in mechanically active tissues like breast glands. In spite of this, so far relatively little attention has been dedicated to their analysis due to the difficulty of isolating and handling such thin layers of material. Here, we isolated basement membranes derived from MCF10A spheroids - 3D breast glands model systems mimicking in-vitro the most relevant phenotypic characteristics of human breast lobules - and characterized them by atomic force microscopy (AFM), enhanced resolution confocal microscopy (LSM), and scanning electron microscopy (SEM). By performing AFM height-clamp experiments, we obtained force relaxation curves that offered the first biomechanical data on isolated breast gland BMs. Based on LSM and SEM imaging data, we modeled the system as a polymer network immersed in liquid and described it as a poroelastic material. Finite Element (FE) simulations matching the experimental force relaxation curves allowed for the first quantification of the bulk and shear moduli of the membrane, as well as its water permeability. These results represent a first step towards a deeper understanding of the mechanism of tensional homeostasis regulating mammary gland activity, as well as its disruption during processes of membrane breaching and metastatic invasion. |
| نوع الوثيقة: | Working Paper |
| DOI: | 10.1016/j.bpj.2018.09.020 |
| URL الوصول: | http://arxiv.org/abs/1811.11110 |
| رقم الانضمام: | edsarx.1811.11110 |
| قاعدة البيانات: | arXiv |
| DOI: | 10.1016/j.bpj.2018.09.020 |
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