Report
Sedimentation of rapidly interacting multicomponent systems
| العنوان: | Sedimentation of rapidly interacting multicomponent systems |
|---|---|
| المؤلفون: | Schuck, Peter, Chaturvedi, Sumit K. |
| سنة النشر: | 2017 |
| المجموعة: | Condensed Matter Physics (Other) Quantitative Biology |
| مصطلحات موضوعية: | Quantitative Biology - Quantitative Methods, Condensed Matter - Soft Condensed Matter, Physics - Biological Physics |
| الوصف: | The biophysical analysis of dynamically formed multi-protein complexes in solution presents a formidable technical challenge. Sedimentation velocity (SV) analytical ultracentrifugation achieves strongly size-dependent hydrodynamic resolution of different size species, and can be combined with multi-component detection by exploiting different spectral properties or temporally modulated signals from photoswitchable proteins. Coexisting complexes arising from self- or hetero-associations that can be distinguished in SV allow measurement of their stoichiometry, affinity, and cooperativity. However, assemblies that are short-lived on the time-scale of sedimentation (t1/2 < 100 sec) will exhibit an as of yet unexplored pattern of sedimentation boundaries governed by coupled co-migration of the entire system. Here, we present a theory for multi-component sedimentation of rapidly interacting systems, which reveals simple underlying physical principles, offers a quantitative framework for analysis, thereby extending the dynamic range of SV for studying multi-component interactions Comment: 18 pages |
| نوع الوثيقة: | Working Paper |
| URL الوصول: | http://arxiv.org/abs/1704.03002 |
| رقم الانضمام: | edsarx.1704.03002 |
| قاعدة البيانات: | arXiv |
| الوصف غير متاح. |