Report
Cross-correlated TIRF/AFM shows Self-assembled Synthetic Myosin Filaments are Asymmetric - Implications for Motile Filaments
| العنوان: | Cross-correlated TIRF/AFM shows Self-assembled Synthetic Myosin Filaments are Asymmetric - Implications for Motile Filaments |
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| المؤلفون: | Brown, Andre E. X., Hategan, Alina, Safer, Daniel, Goldman, Yale E., Discher, Dennis E. |
| سنة النشر: | 2008 |
| المجموعة: | Quantitative Biology |
| مصطلحات موضوعية: | Quantitative Biology - Biomolecules, Quantitative Biology - Quantitative Methods |
| الوصف: | Myosin-II's rod-like tail drives filament assembly with a head arrangement that should generate equal and opposite contractile forces on actin--if one assumes that the filament is a symmetric bipole. Self-assembled myosin filaments are shown here to be asymmetric in physiological buffer based on cross-correlated images from both atomic force microscopy (AFM) and total internal reflection fluorescence (TIRF). Quantitative cross-correlation of these orthogonal methods produces structural information unavailable to either method alone in showing that fluorescence intensity along the filament length is proportional to height. This implies that myosin heads form a shell around the filament axis, consistent with F-actin binding. A motor density of ~50 - 100 heads/micron is further estimated but with an average of 32% more motors on one half of any given filament compared to the other, regardless of length. A purely entropic pyramidal lattice model is developed that qualitatively captures this lack of length dependence and the distribution of filament asymmetries. Such strongly asymmetric bipoles are likely to produce an imbalanced contractile force in cells and in actin-myosin gels, and thereby contribute to motility as well as cytoskeletal tension. Comment: 25 pages, 8 figures. Accepted in Biophysical Journal |
| نوع الوثيقة: | Working Paper |
| URL الوصول: | http://arxiv.org/abs/0811.3040 |
| رقم الانضمام: | edsarx.0811.3040 |
| قاعدة البيانات: | arXiv |
| الوصف غير متاح. |