Ku80 plays a role in non-homologous recombination but is not required for T-DNA integration in Arabidopsis
| العنوان: | Ku80 plays a role in non-homologous recombination but is not required for T-DNA integration in Arabidopsis |
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| المؤلفون: | Gallego, ME, Bleuyard, JY, Daoudal-Cotterell, S, Jallut, N, White, CI |
| المساهمون: | White, Charles, Génétique, Reproduction et Développement (GReD), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Institut National de la Santé et de la Recherche Médicale (INSERM) |
| المصدر: | Plant Journal Plant Journal, 2003, 35 (5), pp.557-65 Plant Journal, Wiley, 2003, 35 (5), pp.557-65 |
| بيانات النشر: | HAL CCSD, 2003. |
| سنة النشر: | 2003 |
| مصطلحات موضوعية: | DNA, Bacterial, MESH: Mutation, DNA Repair, DNA, Plant, Arabidopsis, MESH: DNA Helicases, MESH: Methyl Methanesulfonate, [SDV.GEN] Life Sciences [q-bio]/Genetics, MESH: Arabidopsis Proteins, MESH: Transformation, Genetic, Transformation, Genetic, MESH: Arabidopsis, MESH: DNA, Plant, Recombination, Genetic, MESH: DNA Damage, MESH: DNA Repair, [SDV.GEN]Life Sciences [q-bio]/Genetics, Arabidopsis Proteins, fungi, DNA Helicases, food and beverages, Methyl Methanesulfonate, MESH: DNA, Bacterial, MESH: Tandem Repeat Sequences, Agrobacterium tumefaciens, Tandem Repeat Sequences, MESH: Rhizobium radiobacter, Mutation, MESH: Recombination, Genetic, DNA Damage |
| الوصف: | International audience; Chromosomal breaks are repaired by homologous recombination (HR) or non-homologous end joining (NHEJ) mechanisms. The Ku70/Ku80 heterodimer binds DNA ends and plays roles in NHEJ and telomere maintenance in organisms ranging from yeast to humans. We have previously identified a ku80 mutant of the model plant Arabidopsis thaliana and shown the role of Ku80 in telomere homeostasis in plant cells. We show here that this mutant is hypersensitive to the DNA-damaging agent methyl methane sulphonate and has a reduced capacity to carry out NHEJ recombination. To understand the interplay between HR and NHEJ in plants, we measured HR in the absence of Ku80. We find that the frequency of intrachromosomal HR is not affected by the absence of Ku80. Previous work has clearly implicated the Ku heterodimer in Agrobacterium-mediated T-DNA transformation of yeast. Surprisingly, ku80 mutant plants show no defect in the efficiency of T-DNA transformation of plants with Agrobacterium, showing that an alternative pathway must exist in plants. |
| اللغة: | English |
| تدمد: | 0960-7412 1365-313X |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=pmid_dedup__::f1a2dca2ec1e831636fe86b704f0616c https://www.hal.inserm.fr/inserm-00595815 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.pmid.dedup....f1a2dca2ec1e831636fe86b704f0616c |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 09607412 1365313X |
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