Effects of topically applied tocotrienol on cataractogenesis and lens redox status in galactosemic rats
| العنوان: | Effects of topically applied tocotrienol on cataractogenesis and lens redox status in galactosemic rats |
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| المؤلفون: | Abdul Nasir, N. A., Agarwal, R., Vasudevan, S., Tripathy, M., Renad Alyautdin, Mohd Ismail, N. |
| المصدر: | Scopus-Elsevier Molecular Vision |
| مصطلحات موضوعية: | Galactosemias, Superoxide Dismutase, Viscosity, Administration, Topical, Tocotrienols, Static Electricity, Nitric Oxide Synthase Type II, Catalase, Glutathione, Cataract, Rats, Rats, Sprague-Dawley, Anterior Eye Segment, Stress, Physiological, Malondialdehyde, Lens, Crystalline, Liposomes, Disease Progression, Animals, Tyrosine, Emulsions, Particle Size, Eye Proteins, Oxidation-Reduction, Research Article |
| الوصف: | Purpose Oxidative and nitrosative stress underlies cataractogenesis, and therefore, various antioxidants have been investigated for anticataract properties. Several vitamin E analogs have also been studied for anticataract effects due to their antioxidant properties; however, the anticataract properties of tocotrienols have not been investigated. In this study, we investigated the effects of topically applied tocotrienol on the onset and progression of cataract and lenticular oxidative and nitrosative stress in galactosemic rats. Methods In the first part of this study, we investigated the effects of topically applied microemulsion formulation of tocotrienol (TTE) using six concentrations ranging from 0.01% to 0.2%. Eight groups of Sprague-Dawley rats (n = 9) received distilled water, vehicle, or one of the six TTE concentrations as pretreatment topically twice daily for 3 weeks while on a normal diet. After pretreatment, animals in groups 2–8 received a 25% galactose diet whereas group 1 continued on the normal diet for 4 weeks. During this 4-week period, topical treatment continued as for pretreatment. Weekly slit-lamp examination was conducted to assess cataract progression. At the end of the experimental period, the animals were euthanized, and the proteins and oxidative stress parameters were estimated in the lenses. In the second part of the study, we compared the anticataract efficacy of the TTE with the liposomal formulation of tocotrienol (TTL) using five groups of Sprague-Dawley rats (n = 15) that received distilled water, TTE, TTL, or corresponding vehicle. The mode of administration and dosing schedule were the same as in study 1. Weekly ophthalmic examination and lens protein and oxidative stress estimates were performed as in study 1. Lens nitrosative stress was also estimated. Results During the 4-week treatment period, the groups treated with 0.03% and 0.02% tocotrienol showed slower progression of cataract compared to the vehicle-treated group (p |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=pmid_dedup__::85591f8c745c0d75dbd52f3b7216bff9 http://www.scopus.com/inward/record.url?eid=2-s2.0-84902343353&partnerID=MN8TOARS |
| Rights: | OPEN |
| رقم الانضمام: | edsair.pmid.dedup....85591f8c745c0d75dbd52f3b7216bff9 |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |