One Health compartmental analysis of ESBL-producing Escherichia coli on Reunion Island reveals partitioning between humans and livestock
| العنوان: | One Health compartmental analysis of ESBL-producing Escherichia coli on Reunion Island reveals partitioning between humans and livestock |
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| المؤلفون: | Guillaume, Miltgen, Daniel, Martak, Benoit, Valot, Laure, Kamus, Thomas, Garrigos, Guillaume, Verchere, Houssein, Gbaguidi-Haore, Céline, Ben Cimon, Mahery, Ramiandrisoa, Sandrine, Picot, Anne, Lignereux, Geoffrey, Masson, Marie-Christine, Jaffar-Bandjee, Olivier, Belmonte, Eric, Cardinale, Didier, Hocquet, Patrick, Mavingui, Xavier, Bertrand |
| المساهمون: | Centre hospitalier Félix-Guyon [Saint-Denis, La Réunion], Processus Infectieux en Milieu Insulaire Tropical (PIMIT), Université de La Réunion (UR)-Institut National de la Santé et de la Recherche Médicale (INSERM)-IRD-Centre National de la Recherche Scientifique (CNRS), Laboratoire Chrono-environnement (UMR 6249) (LCE), Centre National de la Recherche Scientifique (CNRS)-Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC), Centre Hospitalier Régional Universitaire de Besançon (CHRU Besançon), Laboratoires Réunilab, Cerballiance, Centre Hospitalier Universitaire de La Réunion (CHU La Réunion), Centre Hospitalier Gabriel Martin, Centre hospitalier de l'Est Réunion, Saint-Benoît, Animal, Santé, Territoires, Risques et Ecosystèmes (UMR ASTRE), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Département Systèmes Biologiques (Cirad-BIOS), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad), This study was supported by Reunion Regional Health Agency (ARS Reunion), Indian Ocean Commission (COI), French Agricultural Research Centre for International Development (CIRAD), UMR PIMIT, through its national institutions (Reunion University, CNRS, INSERM, IRD) and the Biological Resource Centre of Besancon University Hospital. This research was also partially funded by the grants from POE FEDER 2014/2020 of the Conseil Regional de La Reunion (RESISTORUN program). |
| المصدر: | Journal of Antimicrobial Chemotherapy Journal of Antimicrobial Chemotherapy, 2022, 77 (5), pp.1254-1262. ⟨10.1093/jac/dkac054⟩ |
| بيانات النشر: | HAL CCSD, 2022. |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | plasmids, sodium thiosulfate, extended-spectrum beta lactamase, microbial colonization, whole genome sequencing, Livestock, [SDV]Life Sciences [q-bio], reunion island, sequence tagged sites, x-linked, Wastewater, beta-Lactamases, Anti-Bacterial Agents, one health, Animals, Humans, [SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie, ichthyosis, infections, escherichia coli, genes, Reunion, farm animals, Escherichia coli Infections, Multilocus Sequence Typing |
| الوصف: | International audience; Background Extended-spectrum β-lactamase-producing Escherichia coli (ESBL-Ec) is a major cause of infections worldwide. An understanding of the reservoirs and modes of transmission of these pathogens is essential, to tackle their increasing frequency. Objectives We investigated the contributions of various compartments (humans, animals, environment), to human colonization or infection with ESBL-Ec over a 3 year period, on an island. Methods The study was performed on Reunion Island (Southwest Indian Ocean). We collected ESBL-Ec isolates prospectively from humans, wastewater and livestock between April 2015 and December 2018. Human specimens were recovered from a regional surveillance system representative of the island’s health facilities. These isolates were compared with those from livestock and urban/rural wastewater, by whole-genome sequencing. Results We collected 410 ESBL-Ec isolates: 161 from humans, 161 from wastewater and 88 from animals. Phylogenomic analysis demonstrated high diversity (100 STs), with different STs predominating among isolates from humans (ST131, ST38, ST10) and animals (ST57, ST156). The large majority (90%) of the STs, including ST131, were principally associated with a single compartment. The CTX-M-15, CTX-M-27 and CTX-M-14 enzymes were most common in humans/human wastewater, whereas CTX-M-1 predominated in animals. Isolates of human and animal origin had different plasmids carrying blaCTX-M genes, with the exception of a conserved IncI1-ST3 blaCTX-M-1 plasmid.Conclusions These molecular data suggest that, despite their high level of contamination, animals are not a major source of the ESBL-Ec found in humans living on this densely populated high-income island. Public health policies should therefore focus primarily on human-to-human transmission, to prevent human infections with ESBL-Ec. |
| اللغة: | English |
| تدمد: | 0305-7453 1460-2091 |
| DOI: | 10.1093/jac/dkac054⟩ |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=pmid_dedup__::545cb6ea5392419550701d237628f94e https://hal.inrae.fr/hal-03830253 |
| رقم الانضمام: | edsair.pmid.dedup....545cb6ea5392419550701d237628f94e |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 03057453 14602091 |
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| DOI: | 10.1093/jac/dkac054⟩ |