Synthesis of [4S-2H]NADH, [4R-2H]NADH, [4-2H2]NADH and [4-2H]NAD+ cofactors through heterogeneous biocatalysis in heavy water
| العنوان: | Synthesis of [4S-2H]NADH, [4R-2H]NADH, [4-2H2]NADH and [4-2H]NAD+ cofactors through heterogeneous biocatalysis in heavy water |
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| المؤلفون: | Rowbotham, JS, Hardy, AP, Reeve, HA, Vincent, KA |
| المصدر: | Rowbotham, J, Hardy, A, Reeve, H & Vincent, K A 2021, ' Synthesis of [4 S-2 H]NADH, [4 R-2 H]NADH, [4-2 H 2 ]NADH and [4-2 H]NAD + cofactors through heterogeneous biocatalysis in heavy water ', Journal of Labelled Compounds and Radiopharmaceuticals, vol. 64, no. 4, pp. 181-186 . https://doi.org/10.1002/jlcr.3899 Journal of Labelled Compounds & Radiopharmaceuticals |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Practitioner Protocol ‐ Synthesis, dihydrogen gas (H2), H O, Enzymes, Immobilized, NAD, ResearchInstitutes_Networks_Beacons/manchester_institute_of_biotechnology, D O, immobilised enzymes, 2H2O, TheoryofComputation_ANALYSISOFALGORITHMSANDPROBLEMCOMPLEXITY, Manchester Institute of Biotechnology, Biocatalysis, deuterated cofactor, dihydrogen gas (H ), Deuterium Oxide, D2O |
| الوصف: | This practitioner protocol describes the synthesis of a family of deuterated nicotinamide cofactors: [4S‐2H]NADH, [4R‐2H]NADH, [4‐2H2]NADH and [4‐2H]NAD+. The application of a recently developed H2‐driven heterogeneous biocatalyst enables the cofactors to be prepared with high (>90%) 2H‐incorporation with 2H2O as the only isotope source. This protocol describes the use of a H2‐driven heterogeneous biocatalyst to prepare a suite of deuterated nicotinamide cofactors: [4S‐2H]NADH, [4R‐2H]NADH, [4‐2H2]NADH, and [4‐2H]NAD+. The methodology requires only heavy water (2H2O and D2O) as the source of deuterium isotopes. |
| اللغة: | English |
| DOI: | 10.1002/jlcr.3899 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=pmid_dedup__::52cccb0a9a53f9aec65840d919f2e324 https://doi.org/10.1002/jlcr.3899 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.pmid.dedup....52cccb0a9a53f9aec65840d919f2e324 |
| قاعدة البيانات: | OpenAIRE |
| DOI: | 10.1002/jlcr.3899 |
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