To investigate the occurrence of Campylobacter jejuni among other bacterial agents of acute diarrhea.Over a period of 3 months, 114 specimens of acute diarrhea were collected from patients aged 2-58 years referred to Nemazi Hospital, Shiraz, Iran. The samples were tested for bacterial pathogens causing acute diarrhea.Of the 114 samples, 40 proved to be positive for bacterial enteric pathogens: C. jejuni 11 (9.6%), Salmonella spp.18 (15.8%) and Shigella spp. 11 (9.6%). Maximum prevalence of C. jejuni was observed in patients aged 11-15 years. Significantly more females than males were infected with all diarrheal agents (p0.05).This study confirmed the presence of C. jejuni as a significant cause of gastroenteritis in Shiraz, Southwest Iran.