التفاصيل البيبلوغرافية
| العنوان: |
Role and regulation of Glycogen synthase kinase-3 beta in bovine spermatozoa |
| المؤلفون: |
Michael, Belenky, Haim, Breitbart |
| المصدر: |
Molecular reproduction and development. 84(1) |
| سنة النشر: |
2016 |
| مصطلحات موضوعية: |
Enzyme Activation, Male, Sulfonamides, Glycogen Synthase Kinase 3 beta, Acrosome Reaction, Sperm Motility, Animals, Cattle, Phosphorylation, Isoquinolines, Cyclic AMP-Dependent Protein Kinases, Spermatozoa |
| الوصف: |
The serine/threonine kinase Glycogen synthase kinase 3 (GSK-3) is a master switch that regulates a multitude of cellular pathways, including the acrosome reaction in sperm. In epididymal sperm cells, for example, GSK-3 activity correlates with inhibition of motility-yet no direct pathways connecting GSK-3 activation with loss of motility have been described. Indeed, the details of how GSK-3 is regulated during sperm capacitation and the acrosome reaction remains obscure. To this end, we addressed the involvement of the GSK-3 beta isoform in several known pathways that contribute to motility and the acrosome reaction. We established that Protein kinase A (PKA) is the main regulator of GSK-3β in sperm, as pre-treatment of cells with a GSK-3 inhibitor prior to addition of H89, an inhibitor of PKA, attenuated the motility loss induced by blocking PKA activity. Both induced and spontaneous acrosome reactions also occurred less frequently in sperm treated with GSK-3 inhibitors. Finally, we observed a slow decline in phosphorylation of GSK-3β on Ser 9, which represents an inhibited state, during sperm capacitation; this phenotype is reversed during the induced acrosome reaction, in parallel to activation of Protein phosphatase 1. These results suggest that maintenance of sperm motility and acrosome reaction timing are mediated by PKA through the regulation of GSK-3 beta activity. Mol. Reprod. Dev. 84: 8-18, 2017. © 2016 Wiley Periodicals, Inc. |
| تدمد: |
1098-2795 |
| URL الوصول: |
https://explore.openaire.eu/search/publication?articleId=pmid________::e49d48c5cd64a13b4a288d5b59212d85
https://pubmed.ncbi.nlm.nih.gov/27864906 |
| رقم الانضمام: |
edsair.pmid..........e49d48c5cd64a13b4a288d5b59212d85 |
| قاعدة البيانات: |
OpenAIRE |