التفاصيل البيبلوغرافية
| العنوان: |
Simultaneous quantification of major cannabinoids and metabolites in human urine and plasma by HPLC-MS/MS and enzyme-alkaline hydrolysis |
| المؤلفون: |
Oier, Aizpurua-Olaizola, Iratxe, Zarandona, Laura, Ortiz, Patricia, Navarro, Nestor, Etxebarria, Aresatz, Usobiaga |
| المصدر: |
Drug testing and analysis. 9(4) |
| سنة النشر: |
2016 |
| مصطلحات موضوعية: |
Psychotropic Drugs, Cannabinoids, Limit of Detection, Tandem Mass Spectrometry, Solid Phase Extraction, Humans, Dronabinol, Chromatography, High Pressure Liquid |
| الوصف: |
A high performance liquid chromatography coupled to tandem mass spectrometry (HPLC-MS/MS) method for simultaneous quantification of Δ9-tetrahydrocannabinol (THC), its two metabolites 11-hydroxy-Δ9-tetrahydrocannabinol (11-OH-THC) and 11-nor-9-carboxy-Δ9-tetrahydrocannabinol (THC-COOH), and four additional cannabinoids (cannabidiol (CBD), cannabigerol (CBG), tetrahydrocannabivarin (THCV), and cannabinol (CBN)) in 1 mL of human urine and plasma was developed and validated. The hydrolysis process was studied to ensure complete hydrolysis of glucuronide conjugates and the extraction of a total amount of analytes. Initially, urine and plasma blank samples were spiked with THC-COOH-glucuronide and THC-glucuronide, and four different pretreatment methods were compared: hydrolysis-free method, enzymatic hydrolysis with Escherichia Coli β-glucuronidase, alkaline hydrolysis with 10 M NaOH, and enzyme-alkaline tandem hydrolysis. The last approach assured the maximum efficiencies (close to 100%) for both urine and plasma matrices. Regarding the figures of merit, the limits of detection were below 1 ng/mL for all analytes, the accuracy ranged from 84% to 115%, and both within-day and between-day precision were lower than 12%. Finally, the method was successfully applied to real urine and plasma samples from cannabis users. Copyright © 2016 John WileySons, Ltd. |
| تدمد: |
1942-7611 |
| URL الوصول: |
https://explore.openaire.eu/search/publication?articleId=pmid________::cec06f0a6b19d4be05bb408aacdd81f6
https://pubmed.ncbi.nlm.nih.gov/27341312 |
| رقم الانضمام: |
edsair.pmid..........cec06f0a6b19d4be05bb408aacdd81f6 |
| قاعدة البيانات: |
OpenAIRE |