التفاصيل البيبلوغرافية
| العنوان: |
Temporal profiling of the growth and multi-lineage potentiality of adipose tissue-derived mesenchymal stem cells cell-sheets |
| المؤلفون: |
Puay Yong, Neo, Eugene Yong-Shun, See, Siew Lok, Toh, James Cho-Hong, Goh |
| المصدر: |
Journal of tissue engineering and regenerative medicine. 10(7) |
| سنة النشر: |
2012 |
| مصطلحات موضوعية: |
Adipose Tissue, Animals, Cell Differentiation, Core Binding Factor Alpha 1 Subunit, Mesenchymal Stem Cells, SOX9 Transcription Factor, Rabbits, Antigens, Differentiation, Up-Regulation |
| الوصف: |
Cell-sheet tissue engineering retains the benefits of an intact extracellular matrix (ECM) and can be used to produce scaffold-free constructs. Adipose tissue-derived stem cells (ASCs) are multipotent and more easily obtainable than the commonly used bone marrow-derived stem cells (BMSCs). Although BMSC cell sheets have been previously reported to display multipotentiality, a detailed study of the development and multilineage potential of ASC cell sheets (ASC-CSs) is non-existent in the literature. The aims of this study were to temporally profile: (a) the effect of hyperconfluent culture duration on ASC-CSs development; and (b) the multipotentiality of ASC-CSs by differentiation into the osteogenic, adipogenic and chondrogenic lineages. Rabbit ASCs were first isolated and cultured until confluence (day 0). The confluent cells were then cultured in ascorbic acid-supplemented medium for 3 weeks to study cell metabolic activity, cell sheet thickness and early differentiation gene expressions at weekly time points. ASC-CSs and ASCs were then differentiated into the three lineages, using established protocols, and assessed by RT-PCR and histology at multiple time points. ASC-CSs remained healthy up to 3 weeks of hyperconfluent culture. One week-old cell sheets displayed upregulation of early differentiation gene markers (Runx2 and Sox9); however, subsequent differentiation results indicated that they did not necessarily translate to an improved phenotype. ASCs within the preformed cell sheet groups did not differentiate as efficiently as the non-hyperconfluent ASCs, which were directly differentiated. Although ASCs within the cell sheets retained their differentiation capacity and remained viable under prolonged hyperconfluent conditions, future applications of ASC-CSs in tissue engineering should be considered with care. Copyright © 2016 John WileySons, Ltd. |
| تدمد: |
1932-7005 |
| URL الوصول: |
https://explore.openaire.eu/search/publication?articleId=pmid________::4b336ce6243cc78e4516d6fc3bf012ab
https://pubmed.ncbi.nlm.nih.gov/23784965 |
| رقم الانضمام: |
edsair.pmid..........4b336ce6243cc78e4516d6fc3bf012ab |
| قاعدة البيانات: |
OpenAIRE |