Methods for the Extraction of Endosymbionts from the Whitefly Bemisia tabaci

التفاصيل البيبلوغرافية
العنوان: Methods for the Extraction of Endosymbionts from the Whitefly Bemisia tabaci
المؤلفون: Dan-Tong, Zhu, Xin-Ru, Wang, Fei-Xue, Ban, Chi, Zou, Shu-Sheng, Liu, Xiao-Wei, Wang
المصدر: Journal of visualized experiments : JoVE. (124)
سنة النشر: 2017
مصطلحات موضوعية: DNA, Bacterial, Hemiptera, Bacteria, RNA, Ribosomal, 16S, fungi, bacteria, Animals, Metagenome, biochemical phenomena, metabolism, and nutrition, Symbiosis, Polymerase Chain Reaction, Molecular Biology, In Situ Hybridization, Fluorescence
الوصف: Bacterial symbionts form an intimate relationship with their hosts and confer advantages to the hosts in most cases. Genomic information is critical to study the functions and evolution of bacterial symbionts in their host. As most symbionts cannot be cultured in vitro, methods to isolate an adequate quantity of bacteria for genome sequencing are very important. In the whitefly Bemisia tabaci, a number of endosymbionts have been identified and are predicted to be of importance in the development and reproduction of the pests through multiple approaches. However, the mechanism underpinning the associations remains largely unknown. The obstacle partially comes from the fact that the endosymbionts in whitefly, mostly restrained in bacteriocytes, are hard to separate from the host cells. Here we report a step-by-step protocol for the identification, extraction and purification of endosymbionts from the whitefly B. tabaci mainly by dissection and filtration. Endosymbiont samples prepared by this method, although still a mixture of different endosymbiont species, are suitable for subsequent genome sequencing and analysis of the possible roles of endosymbionts in B. tabaci. This method may also be used to isolate endosymbionts from other insects.
تدمد: 1940-087X
URL الوصول: https://explore.openaire.eu/search/publication?articleId=pmid________::30d7cdef761ae3da7d176dfc5cc59fbb
https://pubmed.ncbi.nlm.nih.gov/28654044
Rights: OPEN
رقم الانضمام: edsair.pmid..........30d7cdef761ae3da7d176dfc5cc59fbb
قاعدة البيانات: OpenAIRE