To explicate the operational proportion of public function and items of the centers of disease prevention and control (CDC).161 CDC have been investigated by two-stage stratified sampling to provide the information about the operation of the public function and items of CDC.(1) The average operational proportion investigated CDC is 71.7%, the province level 92.0%, the city level 73.7%, the county level 68.5%. East areas 76.5%, middle areas 71.5%, west areas 68.1%. (2) The operational proportion of disease prevention and control function and emergency treatment function were higher, but that of epidemic situation report and information management of health relative factor, inspection and control of health relative factor, and the laboratory examination and evaluation were lower.Public function and items were rather poorly carried out by the centers of prevention and control in China, especially those in middle and west areas, and those in counties.