Comparison of methods for extraction and purification of DNA from archival material, for use in analysis of forensic samples at the Institute of Research and Expertise in Forensic Genetics
| العنوان: | Comparison of methods for extraction and purification of DNA from archival material, for use in analysis of forensic samples at the Institute of Research and Expertise in Forensic Genetics |
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| المؤلفون: | Netto, Mônica Sertã Corrêa |
| المساهمون: | Silva, Dayse Aparecida da, Garrido, Rodrigo Grazinoli, Tavares, Celso Cruz, Napoleão, Fatima, Sales, Selma Lílian Sallenave, Garrido, Fabíola de Sampaio Rodrigues Grazinoli |
| المصدر: | Biblioteca Digital de Teses e Dissertações da UERJ Universidade do Estado do Rio de Janeiro (UERJ) instacron:UERJ |
| بيانات النشر: | Universidade do Estado do Rio de Janeiro, 2014. |
| سنة النشر: | 2014 |
| مصطلحات موضوعية: | Identificação, DNA extraction protocols, Identificação humana, Genética legal, Forensic genetics, DNA, Patologia forense, Forensic pathology, Protocolos de extração de DNA, Genética forense, Paraffin embedded tissues, Antropologia forense, Human identification, Tecidos em blocos de parafina, CIENCIAS DA SAUDE::MEDICINA::ANATOMIA PATOLOGICA E PATOLOGIA CLINICA [CNPQ] |
| الوصف: | Submitted by Boris Flegr (boris@uerj.br) on 2021-01-07T18:31:49Z No. of bitstreams: 1 Monica Serta Correa Netto Dissertacao completa.pdf: 2408822 bytes, checksum: e9e759042f52d0a0ff66aca01cb330c1 (MD5) Made available in DSpace on 2021-01-07T18:31:49Z (GMT). No. of bitstreams: 1 Monica Serta Correa Netto Dissertacao completa.pdf: 2408822 bytes, checksum: e9e759042f52d0a0ff66aca01cb330c1 (MD5) Previous issue date: 2014-07-28 The DNA fundamented identification is pointed as the biggest revolution in the scientific forensic sphere since the recognition of fingerprints, because of its greatest power of discrimination on the individualizing people. The techniques employed on the DNA identification bear two advantages upon the conventional ones: the chemical stabilization of the DNA, even after a long period of time and its occurrence in all the nucleated cells of the human organism, which allows to convict or acquit a suspect with a drop of blood or an unique hair found in the crime scene. Forensic evidence, from tissues embedded in paraffin archived and safeguarded on The Pathology and Genetics Institute are, in some cases, the only register available of the collected sample. However, there is still a few good protocols in forensic context, to recover DNA from this kind of material, usually from old samples and human remains. The aim of this study is to evaluate methods of extraction and purification of the DNA, using biological archival materials, in order to determine a protocol that offers good recovery of the DNA and adapts on the Institute of Research and Expertise in Forensic Genetics (IPPGF), forensic laboratorial reality and others forensic services. Twenty two samples from cadaver tissues, included in paraffin blocks were submitted to two pre treatment methods: deparaffination using xylene and heating in microwave. After that, four other methods were used for DNA extraction, that includes: Organic Method, Chelex 100®, Purification on membranes (NucleoSpin) and the Resin purification method (DNA IQ). We observed that the spectrofometrical results are not good parameters to support and direct the technical procedures of extraction from the material embedded in paraffin archives, because even when the spectrofometrical results revealed the presence of the DNA, there was no successful amplification of the expected fragment. The polymerase chain reaction demonstrated to be an efficient technique, to amplify a degraded DNA in low quantity. The best DNA concentration for PCR TR were obtained in organic (phenol-chloroform-isoamyl alcohol) and Nucleospin metodologies, been subsequently paired for comparison, using the non parametrical Friedman test, that showed significant differences (p |
| وصف الملف: | application/pdf |
| اللغة: | Portuguese |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=od______3056::9e87ede01fadc375c4d81c44745d76e1 http://www.bdtd.uerj.br/handle/1/15024 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.od......3056..9e87ede01fadc375c4d81c44745d76e1 |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |