Polimorfismos genéticos asociados a enfermedades óseas y fluorosis dental en población expuesta a flúor a través del agua de consumo
| العنوان: | Polimorfismos genéticos asociados a enfermedades óseas y fluorosis dental en población expuesta a flúor a través del agua de consumo |
|---|---|
| المؤلفون: | Jarquín Yáñez, Lizet del Carmen |
| المساهمون: | Mejía Saavedra, José de Jesús, Calderón Hernández, Jaqueline, Alegría Torres, Jorge Alejandro, Consejo Nacional de Ciencia y Tecnología (CONACYT), Fondo sectorial de investigación en salud y seguridad social SS/IMSS/ISSSTE-CONACYT-234310 |
| المصدر: | Universidad Autónoma de San Luis Potosí UASLP Repositorio Institucional de la UASLP |
| بيانات النشر: | Agenda Ambiental, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Hidrofluorosis, Polimorfismos, Densidad mineral ósea, Fluorosis Dental, Estado nutricional, Micronutrientes, Hydrofluorosis, Polymorphisms, Bone mineral density, Dental Fluorosis, Nutritional status, Micronutrients, 3 MEDICINA Y CIENCIAS DE LA SALUD |
| الوصف: | Lack of water has originated the exploitation of aquifers, which, based on the lithological and structural characteristics of the rocks, constitute a natural source of fluoride. Since this type of water is the main source of drinking water supply in several regions, fluoride contamination in water intended for human consumption has become a relevant problem worldwide. The aim of this study was to evaluate the prevalence of genetic polymorphisms and their influence on bone diseases and dental fluorosis in children exposed to fluoride through drinking water in communities of Ojo Caliente, Santa María del Río y La Reforma, Salinas de Hidalgo in the state of San Luis Potosí. To this end, a cross-sectional study was designed in which 241 children were evaluated, 121 from Ojo Caliente and 120 from La Reforma. Samples of drinking water and urine were requested for the quantification of fluoride through the potentiometric method with selective ion electrode. The polymorphisms rs412777 in the COL1A2 gene, rs2234693 in the ESR1 gene and rs1256049 in the ESR2 gene were evaluated through the real-time PCR technique. The value of the bone mineral density (BMD) of the lumbar region (L1-L4) and left hip was evaluated using a double-energy X-ray densitometry (DEXA) device: "Hologic Model Discovery Wi" (S / N 85714) with software for infants and pediatrics, whose results were interpreted in Z values adjusted for age, sex, weight, height and ethnicity. Dental fluorosis was evaluated using the Thylstrup-Fejerskov index (TF) and included the evaluation of the nutritional status based on the World Health Organization (WHO) and the intake of micronutrients obtained through a validated consumption frequency questionnaire, for Mexican population. Likewise, a risk communication program was implemented in the study populations. The concentration of fluoride in the tap water was 2.36 ± 0.02 mg / L in the community of Ojo Caliente and 4.56 ± 0.07 mg / L in the community of La Reforma. While the concentration of fluoride in bottled water was 0.14 ± 0.04 mg / L and 0.47 ± 0.05 mg / L respectively. The calculated risk factor for dental fluorosis was 2.0 for Ojo Caliente and 4.2 for La Reforma since the main source of water consumption was from the tap. The level of fluoride exposure was 2.05 ± 0.62 mg / L in Ojo Caliente and 2.99 ± 0.99 mg / L in La Reforma. There were 218 cases of normal BMD and 23 of low BMD, and 100% of the evaluated population registered dental fluorosis. 67% of the population had a normal nutritional status, 27% underweight, 2% moderate malnutrition and 4% severe malnutrition. A consumption 150% of the RDI for magnesium in children of 7-8 years, 28% in those of 9-13 years and 10% in those of 14-15 years. While for phosphorus 5% of children aged 9-13 years and 10% of those aged 14-15 years had a consumption 150% of RDI. The presence of the polymorphism evaluated in the COL1A2 gene generated an OR = 7.10 (CI: 3.96-12.70) for the children who carried the polymorphism regardless of whether it was homozygous or heterozygous with more severe dental fluorosis (TF6,7,8,9 ), with respect to children who did not take it. Of the 22 cases of low BMD that had the analysis for the identification of the polymorphism, 10 presented the polymorphism (7 cases with A / C and 3 cases with C / C) and 12 did not present it, so no association was found. The levels of exposure to fluoride were lower in children who presented low BMD with respect to those who presented normal BMD (2.12 ± 0.78 v / s 2.56 ± 0.96, p 150 % of the phosphorus IDR had a higher concentration of fluorine in urine compared to those with lower consumption (2.43 ± 0.59 v / s 1.82 ± 0.54 and 1.82 ± 0.13 mg / L, p 150% de la IDR para magnesio en los niños de 7-8 años, el 28% en los de 9-13 años y el 10% en los de 14-15 años. En tanto que para fósforo el 5% de los niños de 9-13 años y el 10% de los de 14-15 años presentaron un consumo 150% de IDR. La presencia del polimorfismo evaluado en el gen COL1A2 generó un OR=7.10 (IC: 3.96-12.70) para los niños que llevaron el polimorfismo independientemente de que este fuera homocigoto o heterocigoto de presentar fluorosis dental más severa (TF6,7,8,9), con respecto a los niños que no lo llevaron. De los 22 casos de DMO baja que contaron con el análisis para la identificación del polimorfismo, 10 presentaron el polimorfismo (7 casos con A/C y 3 casos con C/C) y 12 no lo presentaron, por lo que no se encontró asociación. Los niveles de exposición a flúor fueron menores en los niños que presentaron DMO baja con respecto a los que presentaron DMO normal (2.12 ± 0.78 v/s 2.56 ± 0.96, p150% de la IDR de fósforo tuvieron una concentración mayor de flúor en orina con respecto a los que tuvieron un consumo menor (2.43 ± 0.59 v/s 1.82 ± 0.54 y 1.82 ± 0.13 mg/L, p |
| وصف الملف: | application/pdf |
| اللغة: | Spanish; Castilian |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=od______3056::9b87e7278c32874cea1323e8a7ab4432 https://repositorioinstitucional.uaslp.mx/xmlui/handle/i/4731 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.od......3056..9b87e7278c32874cea1323e8a7ab4432 |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |