Mechanism of generation of humoral immune response induced by targeting of MSP119PADRE antigen to two different subsets of dendritic cells via DEC205 and DCIR2 receptors
| العنوان: | Mechanism of generation of humoral immune response induced by targeting of MSP119PADRE antigen to two different subsets of dendritic cells via DEC205 and DCIR2 receptors |
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| المؤلفون: | Fernando Bandeira Sulczewski |
| المساهمون: | Silvia Beatriz Boscardin, Daniela Santoro Rosa, Jose Alexandre Marzagao Barbuto, Alexandre de Castro Keller, Maria Regina D'Imperio Lima |
| المصدر: | Biblioteca Digital de Teses e Dissertações da USP Universidade de São Paulo (USP) instacron:USP |
| بيانات النشر: | Universidade de São Paulo, 2017. |
| سنة النشر: | 2017 |
| الوصف: | As células dendríticas (DCs) são células do sistema imune inato que são especializadas na instrução de repostas imunes adaptativas No baço murinho, as DCs convencionais podem ser classificadas em CD8α+ DEC205+ e CD8α- DCIR2+. Anticorpos monoclonais (mAbs) αDEC205 e αDCIR2 (33D1) conjugados a proteínas antigênicas e são utilizados como estratégia de direcionamento de antígenos para as DCs CD8α+ e CD8α-. Foi utilizado o antígeno quimérico MSP119PADRE conjugado aos mAbs αDEC205 e αDCIR2 e o Poly I:C como adjuvante. A montagem da resposta celular e humoral foi analisada 3, 4, 5 e 6 dias após primeira e a segunda As DCS CD8α- são especializadas na instrução de células TFH. Porém, num segundo dose de mAbs há a instrução de uma resposta Th2/Th17. E, o direcionamento de antígenos para DCS CD8α+ induz uma resposta Th1, indicando que essas células são especializadas na instrução desse perfil de resposta auxiliar. Apesar disso, na segunda imunização há a diferenciação de células TFH. As dendritic cells (DC) are innate immune cells that are specialized to prime adaptive immune cells. In the murine spleen, conventional DCs can be classified into CD8α+ DEC205+ and CD8α-DCIR2+. Monoclonal antibodies (mAbs) αDEC205 and αDCIR2 (33D1) conjugated to antigenic proteins have benn used as antigen targeting strategy to CD8α+ and CD8α- DCs. MSP119PADRE chimeric antigen conjugated to mAbs αDEC205 and αDCIR2 and Poly I: C as adjuvant was used. The assembly of the cellular and humoral response was analyzed 3, 4, 5 and 6 days after the first and second doses of imnunization. DCs CD8α- are specialized in the instruction of TFH cells. However, there is an a promotion of Th1 response by CD8α+, indicating that these cells are specialized in the instruction of the helper response profile. Nevertheless, in the second immunization there is a differentiation of TFH cells. |
| اللغة: | Portuguese |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=od______3056::18912492b00da5deb3b54c0da6992c51 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.od......3056..18912492b00da5deb3b54c0da6992c51 |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |