Animal models are widely used for research of liver diseases pathogenesis and progression and for development of new treatment strategies in hepatology. The dissertation thesis focuses on large animal model, specifically swine. The use of animals, which are anatomically and physiologically close to humans, allows us to bridge the gap between the experimental and human medicine. Histopathological analysis of the liver biopsies is still a fundamental part of liver disease diagnosis and therefore, it is also a part of the experimental design of the studies using the porcine liver. Our aim was to apply qualitative and quantitative histological methods of evaluation on porcine liver and to assess their usability in experimental medicine. The quantitative methods included automated image analysis as well as stereological methods, which guaranteed high reproducibility and comparability of the experimental results. The dissertation thesis is based on 10 manuscripts. Three of them are published reviews associated with the main topic of the thesis. Seven original manuscripts resulted from six experimental studies - their six conclusions are listed bellow: Conclusion 1: We developed an open-source software QuantAn for quantification of microvessels visualized by 3D imaging methods, such as computed tomography...