Control of Protein Affinity of Bioactive Nanocellulose and Passivation Using Engineered Block and Random Copolymers
| العنوان: | Control of Protein Affinity of Bioactive Nanocellulose and Passivation Using Engineered Block and Random Copolymers |
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| المؤلفون: | Orlando J. Rojas, Leena-Sisko Johansson, Hannes Orelma, Maija Vuoriluoto, Baolei Zhu |
| المصدر: | Vuoriluoto, M, Orelma, H, Zhu, B, Johansson, L S & Rojas, O J 2016, ' Control of Protein Affinity of Bioactive Nanocellulose and Passivation Using Engineered Block and Random Copolymers ', ACS Applied Materials & Interfaces, vol. 8, no. 8, pp. 5668-5678 . https://doi.org/10.1021/acsami.5b11737 |
| بيانات النشر: | American Chemical Society (ACS), 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | Staphylococcus aureus, PDMAEMA, Materials science, Surface Properties, biosurfaces, ta221, Nanofibers, Biocompatible Materials, Ether, Biointerface, 02 engineering and technology, 010402 general chemistry, Methacrylate, 01 natural sciences, TEMPO-oxidation, Nanocellulose, chemistry.chemical_compound, nonspecific adsorption, Polymer chemistry, Copolymer, Humans, General Materials Science, Cellulose, Staphylococcal Protein A, ta216, Aqueous solution, ta213, biology, antifouling, cellulose nanofibrils, Water, POEGMA, 021001 nanoscience & nanotechnology, 0104 chemical sciences, Nylons, chemistry, Immunoglobulin G, biology.protein, Methacrylates, human IgG, 0210 nano-technology, Protein A, Ethylene glycol |
| الوصف: | We passivated TEMPO-oxidized cellulose nanofibrils (TOCNF) toward human immunoglobulin G (hIgG) by modification with block and random copolymers of poly(2-(dimethylamino)ethyl methacrylate) (PDMAEMA) and poly(oligo(ethylene glycol) methyl ether methacrylate) (POEGMA). The block copolymers reversibly adsorbed on TOCNF and were highly effective in preventing nonspecific interactions with hIgG, especially if short PDMAEMA blocks were used. In such cases, total protein rejection was achieved. This is in contrast to typical blocking agents, which performed poorly. When an anti-human IgG biointerface was installed onto the passivated TOCNF, remarkably high affinity antibody-antigen interactions were observed (0.90 ± 0.09 mg/m(2)). This is in contrast to the nonpassivated biointerface, which resulted in a significant false response. In addition, regeneration of the biointerface was possible by low pH aqueous wash. Protein A from Staphylococcus aureus was also utilized to successfully increase the sensitivity for human IgG recognition (1.28 ± 0.11 mg/m(2)). Overall, the developed system based on TOCNF modified with multifunctional polymers can be easily deployed as bioactive material with minimum fouling and excellent selectivity. |
| تدمد: | 1944-8252 1944-8244 |
| DOI: | 10.1021/acsami.5b11737 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ffafd4352a9aad1b5df5e05d7a1d58d7 https://doi.org/10.1021/acsami.5b11737 |
| Rights: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....ffafd4352a9aad1b5df5e05d7a1d58d7 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19448252 19448244 |
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| DOI: | 10.1021/acsami.5b11737 |