Phenolic profile, antioxidant capacity and antimicrobial activity of nettle leaves extracts obtained by advanced extraction techniques
| العنوان: | Phenolic profile, antioxidant capacity and antimicrobial activity of nettle leaves extracts obtained by advanced extraction techniques |
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| المؤلفون: | Verica Dragović-Uzelac, Sonja Smole Možina, Maja Repajić, Valentina Malin, Zoran Zorić, Ivona Elez Garofulić, Sandra Pedisić, Meta Sterniša |
| المصدر: | Molecules, vol. 26, no. 20, 6153, 2021. Molecules Molecules, Vol 26, Iss 6153, p 6153 (2021) Volume 26 Issue 20 |
| بيانات النشر: | MDPI, 2022. |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | Antioxidant, medicine.medical_treatment, Pharmaceutical Science, antioxidant capacity, High-performance liquid chromatography, Antioxidants, Analytical Chemistry, UPLC MS2, chemistry.chemical_compound, Ingredient, QD241-441, Anti-Infective Agents, Pseudomonas fragi, Drug Discovery, pressurized liquid extraction, Food science, Microwaves, Chromatography, High Pressure Liquid, biology, Chemistry, protimikrobno delovanje, Urtica dioica, microwave-assisted extraction, Antimicrobial, Anti-Bacterial Agents, nettle leaves, advanced extraction techniques, plant polyphenols, ORAC, antimicrobial activity, rastlinski izvlečki, Chemistry (miscellaneous), Molecular Medicine, fenolne spojine, Article, ekstrakcijske metode, Phenols, kopriva, medicine, Physical and Theoretical Chemistry, udc:547.9:582.635.8:66.061, UPLC MS$^2$, Flavonoids, ekstrakcija, Plant Extracts, Organic Chemistry, Extraction (chemistry), antioksidativni potencial, biology.organism_classification, Plant Leaves, Polyphenol |
| الوصف: | Nettle is a widely known plant whose high biological activity and beneficial medicinal effects are attributed to various bioactive compounds, among which polyphenols play an important role. In order to isolate polyphenols and preserve their properties, advanced extraction techniques have been applied to overcome the drawbacks of conventional ones. Therefore, microwave-assisted extraction (MAE) has been optimized for the isolation of nettle leaves polyphenols and it was compared to pressurized liquid extraction (PLE) and conventional heat-reflux extraction (CE). The obtained extracts were analyzed for their individual phenolic profile by UPLC MS2 and for their antioxidant capacity by ORAC assay. MAE proved to be the more specific technique for the isolation of individual phenolic compounds, while PLE produced extracts with higher amount of total phenols and higher antioxidant capacity. Both techniques were more effective compared to CE. PLE nettle extract showed antimicrobial activity against bacteria, especially against Gram-negative Pseudomonas fragi ATCC 4973 and Campylobacter jejuni NCTC 11168 strains. This suggests that PLE is suitable for obtaining a nettle extract with antioxidant and antimicrobial potential, which as such has great potential for use as a value-added ingredient in the food and pharmaceutical industry. |
| وصف الملف: | application/pdf; text/url |
| اللغة: | English |
| تدمد: | 1420-3049 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fee0a4f9d3b64eb6f61a6f729a9a60df https://hdl.handle.net/20.500.12556/RUL-136263 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....fee0a4f9d3b64eb6f61a6f729a9a60df |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14203049 |
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