Proteomic analysis of PTCH1 +/- Fibroblast lysate and conditioned culture media isolated from the skin of healthy subjects and nevoid basal cell carcinoma syndrome patients
| العنوان: | Proteomic analysis of PTCH1 +/- Fibroblast lysate and conditioned culture media isolated from the skin of healthy subjects and nevoid basal cell carcinoma syndrome patients |
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| المؤلفون: | Lorenza Pastorino, Paola Azzoni, Elisa Bellei, Stefania Bergamini, Aurora Cuoghi, Giovanni Pellacani, Cristina Magnoni, Emanuela Monari, Annamaria Pollio, Tiziana Petrachi, Giorgia Bertazzoni, Luisa Benassi, Alexander Witkowski, Pietro Loschi, Aldo Tomasi, Giovanni Ponti |
| المصدر: | BioMed Research International BioMed Research International, Vol 2013 (2013) |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | Patched Receptors, Proteomics, Genetics and Molecular Biology (all), Pathology, medicine.medical_specialty, endocrine system, Skin Neoplasms, MMP1, Article Subject, Immunology and Microbiology (all), Basal Cell Nevus Syndrome, lcsh:Medicine, Nevoid basal-cell carcinoma syndrome, Context (language use), Receptors, Cell Surface, Biology, Culture Media, Conditioned, Fibroblasts, Humans, Mutation, Patched-1 Receptor, Biochemistry, Genetics and Molecular Biology (all), Biochemistry, General Biochemistry, Genetics and Molecular Biology, Pathogenesis, Conditioned, proteomic analysis, Fibroblast Lysate and Conditioned Culture Media, Receptors, medicine, Fibroblast, Proteomic Profile, General Immunology and Microbiology, lcsh:R, General Medicine, medicine.disease, Culture Media, medicine.anatomical_structure, PTCH1, Cell Surface, Research Article |
| الوصف: | Background. The pathogenesis underlying the increased predisposition to the development of basal cell carcinomas (BCCs) in the context of Gorlin-Goltz syndrome is linked to molecular mechanisms that differ from sporadic BCCs. Patients with Gorlin syndrome tend to develop multiple BCCs at an early age and present with tumors of non-sun-exposed skin. The aim of this study was to compare the proteomic profile of cultured fibroblast and fibroblast conditioned culture media of PTCH1+ and nonmutated fibroblasts. Results. Proteomic analysis was performed using Surface-Enhanced Laser Desorption/Ionization Time-of-Flight mass spectrometry in PTCH1+ fibroblast conditioned media isolated from not affected sun-protected skin areas of Gorlin patients and from healthy subjects. 12 protein cluster peaks, >5 kDa, had significant differences in their peak intensities between PTCH1+ and PTCH1− subject groups. We detected a strongly MMP1 overexpression in PTCH1+ fibroblasts obtained from NBCCS patients with respect to healthy donors. Conclusion. Protein profiles in the fibroblast conditioned media revealed statistically significant differences between two different types (missense versus nonsense) of PTCH1 mutations. These differences could be useful as signatures to identify PTCH1 gene carriers at high risk for the development of NBCCS-associated malignancies and to develop novel experimental molecular tailored therapies based on these druggable targets. |
| وصف الملف: | text/xhtml |
| اللغة: | English |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::febbb0e71493323c8e93e9be1d3750c1 http://hdl.handle.net/11567/942548 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....febbb0e71493323c8e93e9be1d3750c1 |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |