IL-17A/F-signaling does not contribute to the initial phase of mucosal inflammation triggered by S. Typhimurium
| العنوان: | IL-17A/F-signaling does not contribute to the initial phase of mucosal inflammation triggered by S. Typhimurium |
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| المؤلفون: | Laurye Van Maele, Jean-Claude Sirard, Till A. Röhn, Manfred Kopf, Pascal Songhet, Delphine Cayet, Manja Barthel, Wolf-Dietrich Hardt, Martin F. Bachmann |
| المساهمون: | Eidgenössische Technische Hochschule - Swiss Federal Institute of Technology [Zürich] (ETH Zürich), Cytos Biotechnology, Cytos Biotechnology, AG, Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 (CIIL), Centre National de la Recherche Scientifique (CNRS)-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-Université de Lille-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP), Institute of Integrative Biology, Molecular Biomedicine, Grants to WDH from the Swiss National Science Foundation (310000-113623/1 and 310030-132997/1), the KTI (to MB andWDH) and a grant to JCS and WDH from the European Union (SavinMucoPath INCO-CT-2006-032296)., Sirard, Jean-Claude, Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-Centre National de la Recherche Scientifique (CNRS) |
| المصدر: | PLoS ONE PLoS ONE, Public Library of Science, 2010, 5 (11), pp.e13804. ⟨10.1371/journal.pone.0013804⟩ PloS one PLoS ONE, 2010, 5 (11), pp.e13804. ⟨10.1371/journal.pone.0013804⟩ PLoS ONE, 5 (11) PLoS ONE, Vol 5, Iss 11, p e13804 (2010) |
| بيانات النشر: | HAL CCSD, 2010. |
| سنة النشر: | 2010 |
| مصطلحات موضوعية: | Male, Salmonella typhimurium, MESH: Signal Transduction, Chemokine, Time Factors, medicine.medical_treatment, MESH: Interleukin-17, MESH: Receptors, Interleukin-17, MESH: Mice, Knockout, MESH: Enterocolitis, Type three secretion system, Infectious Diseases/Bacterial Infections, Mice, 0302 clinical medicine, Intestinal mucosa, MESH: Reverse Transcriptase Polymerase Chain Reaction, MESH: Animals, Cecum, Mice, Knockout, 0303 health sciences, MESH: Cytokines, Multidisciplinary, Receptors, Interleukin-17, biology, Effector, Reverse Transcriptase Polymerase Chain Reaction, Interleukin-17, Vaccination, MESH: Chemokines, 3. Good health, CXCL1, Cytokine, Cytokines, Medicine, [SDV.IMM]Life Sciences [q-bio]/Immunology, Female, Interleukin 17, medicine.symptom, Chemokines, Signal Transduction, Research Article, MESH: Mutation, MESH: Salmonella Infections, Animal, [SDV.IMM] Life Sciences [q-bio]/Immunology, Science, Inflammation, Microbiology, 03 medical and health sciences, MESH: Gene Expression Profiling, MESH: Mice, Inbred C57BL, Immunology/Immunity to Infections, medicine, Animals, MESH: Mice, 030304 developmental biology, Salmonella Infections, Animal, Enterocolitis, Gene Expression Profiling, MESH: Time Factors, MESH: Cecum, MESH: Salmonella typhimurium, MESH: Vaccination, MESH: Male, Mice, Inbred C57BL, Immunology, Mutation, Immunology/Immune Response, biology.protein, MESH: Female, 030215 immunology |
| الوصف: | International audience; Salmonella enterica subspecies 1 serovar Typhimurium (S. Typhimurium) causes diarrhea and acute inflammation of the intestinal mucosa. The pro-inflammatory cytokines IL-17A and IL-17F are strongly induced in the infected mucosa but their contribution in driving the tissue inflammation is not understood. We have used the streptomycin mouse model to analyze the role of IL-17A and IL-17F and their cognate receptor IL-17RA in S. Typhimurium enterocolitis. Neutralization of IL-17A and IL-17F did not affect mucosal inflammation triggered by infection or spread of S. Typhimurium to systemic sites by 48 h p.i. Similarly, Il17ra(-/-) mice did not display any reduction in infection or inflammation by 12 h p.i. The same results were obtained using S. Typhimurium variants infecting via the TTSS1 type III secretion system, the TTSS1 effector SipA or the TTSS1 effector SopE. Moreover, the expression pattern of 45 genes encoding chemokines/cytokines (including CXCL1, CXCL2, IL-17A, IL-17F, IL-1α, IL-1β, IFNγ, CXCL-10, CXCL-9, IL-6, CCL3, CCL4) and antibacterial molecules was not affected by Il17ra deficiency by 12 h p.i. Thus, in spite of the strong increase in Il17a/Il17f mRNA in the infected mucosa, IL-17RA signaling seems to be dispensable for eliciting the acute disease. Future work will have to address whether this is attributable to redundancy in the cytokine signaling network. |
| وصف الملف: | application/pdf; application/application/pdf |
| اللغة: | English |
| تدمد: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0013804⟩ |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fea140f283dffad46047de4147bb2983 https://www.hal.inserm.fr/inserm-00926613/document |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....fea140f283dffad46047de4147bb2983 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19326203 |
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| DOI: | 10.1371/journal.pone.0013804⟩ |