Visualization by High Resolution Immunoelectron Microscopy of the Transient Receptor Potential Vanilloid-1 at Inhibitory Synapses of the Mouse Dentate Gyrus
| العنوان: | Visualization by High Resolution Immunoelectron Microscopy of the Transient Receptor Potential Vanilloid-1 at Inhibitory Synapses of the Mouse Dentate Gyrus |
|---|---|
| المؤلفون: | Inmaculada Gerrikagoitia, Nagore Puente, Leire Reguero, Miren-Josune Canduela, Almudena Ramos-Uriarte, Juan Mendizabal-Zubiaga, Izaskun Elezgarai, Pedro Grandes |
| المصدر: | Addi. Archivo Digital para la Docencia y la Investigación instname PLoS ONE, Vol 10, Iss 3, p e0119401 (2015) PLoS ONE |
| بيانات النشر: | Public Library Science, 2015. |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | Male, medicine.medical_specialty, mice, hypocampus, BIOCHEMISTRY AND MOLECULAR BIOLOGY, TRPV1 channels, Immunoelectron microscopy, brain, TRPV Cation Channels, lcsh:Medicine, Biology, Hippocampal formation, Gene Knockout Techniques, Postsynaptic potential, Internal medicine, expression, medicine, Animals, endocannabinoid system, Long-term depression, Microscopy, Immunoelectron, lcsh:Science, long term depression, Multidisciplinary, synaptic plasticity, MEDICINE, Dentate gyrus, musculoskeletal, neural, and ocular physiology, lcsh:R, cannabinoid receptor, Dendrites, Perforant path, Endocrinology, medicine.anatomical_structure, Inhibitory Postsynaptic Potentials, nervous system, immunohistochemical localization, AGRICULTURAL AND BIOLOGICAL SCIENCES, Cell Body, Synaptic plasticity, Dentate Gyrus, Synapses, Excitatory postsynaptic potential, Biophysics, Female, lipids (amino acids, peptides, and proteins), lcsh:Q, Research Article |
| الوصف: | We have recently shown that the transient receptor potential vanilloid type 1 (TRPV1), a non-selective cation channel in the peripheral and central nervous system, is localized at postsynaptic sites of the excitatory perforant path synapses in the hippocampal dentate molecular layer (ML). In the present work, we have studied the distribution of TRPV1 at inhibitory synapses in the ML. With this aim, a preembedding immunogold method for high resolution electron microscopy was applied to mouse hippocampus. About 30% of the inhibitory synapses in the ML are TRPV1 immunopositive, which is mostly localized perisynaptically (similar to 60% of total immunoparticles) at postsynaptic dendritic membranes receiving symmetric synapses in the inner 1/3 of the layer. This TRPV1 pattern distribution is not observed in the ML of TRPV1 knock-out mice. These findings extend the knowledge of the subcellular localization of TRPV1 to inhibitory synapses of the dentate molecular layer where the channel, in addition to excitatory synapses, is present. P. Grandes' laboratory is provided by Ministerio de Economia y Competitividad (BFU2012-33334); Basque Government grant IT764-13; University of the Basque Country (UFI11/41) and by Red de Trastornos Adictivos, RETICS, Instituto de Salud Carlos III, grant number: RD07/0001/2001, RD12/0028/0004. |
| وصف الملف: | application/pdf |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fe8975177a858b68dfd60b3b5fa94320 http://hdl.handle.net/10810/16798 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....fe8975177a858b68dfd60b3b5fa94320 |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |