C. pseudotuberculosis Phop confers virulence and may be targeted by natural compounds
| العنوان: | C. pseudotuberculosis Phop confers virulence and may be targeted by natural compounds |
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| المؤلفون: | Vasco Azevedo, Sandeep Tiwari, Fernanda Alves Dorella, Rafael Hirata, Artur Silva, Alberto Oliveira, Syed Babar Jamal, Maria Fátima da Silva Teixeira, Marcília P. Costa, Diana Oliveira, Flávia S. Rocha, Vinicius A. C. Abreu, Sintia Almeida, Edson Luiz Folador, Syed Shah Hassan, Debmalya Barh |
| المصدر: | Integr. Biol.. 6:1088-1099 |
| بيانات النشر: | Oxford University Press (OUP), 2014. |
| سنة النشر: | 2014 |
| مصطلحات موضوعية: | DNA, Bacterial, Cell Survival, Rheum undulatum, Biophysics, Virulence, Anthraquinones, Polymerase Chain Reaction, Biochemistry, Cell Line, Microbiology, Bacterial genetics, Mice, Bacterial Proteins, Animals, Gene, Sequence Deletion, Mice, Inbred BALB C, Corynebacterium Infections, biology, Corynebacterium pseudotuberculosis, Macrophages, Histidine kinase, biochemical phenomena, metabolism, and nutrition, biology.organism_classification, Transmembrane protein, Molecular Docking Simulation, Response regulator, bacteria, Caseous lymphadenitis, Biological Assay, Signal Transduction |
| الوصف: | The bacterial two-component system (TCS) regulates genes that are crucial for virulence in several pathogens. One of such TCS, the PhoPR system, consisting of a transmembrane sensory histidine kinase protein (PhoR) and an intracellular response regulator protein (PhoP), has been reported to have a major role in mycobacterial pathogenesis. We knocked out the phoP in C. pseudotuberculosis, the causal organism of caseous lymphadenitis (CLA), and using a combination of in vitro and in vivo mouse system, we showed for the first time, that the PhoP of C. pseudotuberculosis plays an important role in the virulence and pathogenicity of this bacterium. Furthermore, we modeled the PhoP of C. pseudotuberculosis and our docking results showed that several natural compounds including Rhein, an anthraquinone from Rheum undulatum, and some drug-like molecules may target PhoP to inhibit the TCS of C. pseudotuberculosis, and therefore may facilitate a remarkable attenuation of bacterial pathogenicity being the CLA. Experiments are currently underway to validate these in silico docking results. |
| تدمد: | 1757-9708 1757-9694 |
| DOI: | 10.1039/c4ib00140k |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fd92b91b3a44f9a11eb975d1b1dd2751 https://doi.org/10.1039/c4ib00140k |
| رقم الانضمام: | edsair.doi.dedup.....fd92b91b3a44f9a11eb975d1b1dd2751 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 17579708 17579694 |
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| DOI: | 10.1039/c4ib00140k |