Chlamydomonas carries out fatty acid β-oxidation in ancestral peroxisomes using a bona fide acyl-CoA oxidase
| العنوان: | Chlamydomonas carries out fatty acid β-oxidation in ancestral peroxisomes using a bona fide acyl-CoA oxidase |
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| المؤلفون: | Bertrand Legeret, Audrey Beyly-Adriano, Johnathan A. Napier, Yuanxue Liang, Gilles Peltier, Stéphanie Blangy, Fantao Kong, Fred Beisson, Yonghua Li-Beisson, Richard P. Haslam |
| المساهمون: | Environnement, Bioénergie, Microalgues et Plantes (EBMP), Institut de Biosciences et Biotechnologies d'Aix-Marseille (ex-IBEB) (BIAM), Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Biological Chemistry and Crop Protection, Rothamsted Research, Biotechnology and Biological Sciences Research Council (BBSRC)-Biotechnology and Biological Sciences Research Council (BBSRC), ANR-13-JSV5-0005,MUsCA,Ingénierie métabolique d'une microalgue verte en vue de la production d'alcanes à chaine moyenne(2013), ANR-11-IDEX-0001,Amidex,INITIATIVE D'EXCELLENCE AIX MARSEILLE UNIVERSITE(2011), Bioénergie et Microalgues (EBM), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS) |
| المصدر: | Plant Journal Plant Journal, 2017, 90 (2), pp.358-371. ⟨10.1111/tpj.13498⟩ Plant Journal, Wiley, 2017, 90 (2), pp.358-371. ⟨10.1111/tpj.13498⟩ |
| بيانات النشر: | HAL CCSD, 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | 0106 biological sciences, 0301 basic medicine, Nitrogen, Mutant, Chlamydomonas reinhardtii, lipid droplet, hydrogen peroxide, Plant Science, 01 natural sciences, 03 medical and health sciences, Peroxisomes, Genetics, microbodies, Acyl-CoA oxidase, Microbody, nitrogen starvation, [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], chemistry.chemical_classification, Oxidase test, biology, Chlamydomonas, catalase, acyl-CoA oxidase, Fatty acid, lipid catabolism, Cell Biology, Peroxisome, Lipid Metabolism, biology.organism_classification, lipid homeostasis, 030104 developmental biology, Biochemistry, chemistry, oil content, Oxidation-Reduction, 010606 plant biology & botany |
| الوصف: | International audience; Peroxisomes are thought to have played a key role in the evolution of metabolic networks of photosynthetic organisms by connecting oxidative and biosynthetic routes operating in different compartments. While the various oxidative pathways operating in the peroxisomes of higher plants are fairly well characterized, the reactions present in the primitive peroxisomes (microbodies) of algae are poorly understood. Screening of a Chlamydomonas insertional mutant library identified a strain strongly impaired in oil remobilization and defective in Cre05.g232002(CrACX2), a gene encoding a member of the acyl-CoA oxidase/dehydrogenase superfamily. The purified recombinant CrACX2 expressed in Escherichia coli catalyzed the oxidation of fatty acyl-CoAs into trans-2-enoyl-CoA and produced H 2 O 2. This result demonstrated that CrACX2 is a genuine acyl-CoA oxidase, which is responsible for the first step of the peroxisomal fatty acid (FA) β-oxidation spiral. A fluorescent protein-tagging study pointed to a peroxisomal location of CrACX2. The importance of peroxisomal FA β-oxidation in algal physiology was shown by the impact of the mutation on FA turnover during day/night cycles. Moreover, under nitrogen depletion the mutant accumulated 20% more oil than the wild type, illustrating the potential of β-oxidation mutants for algal biotechnology. This study provides experimental evidence that a plant-type FA β-oxidation involving H 2 O 2-producing acyl-CoA oxidation activity has already evolved in the microbodies of the unicellular green alga Chlamydomonas reinhardtii. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 0960-7412 1365-313X |
| DOI: | 10.1111/tpj.13498⟩ |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fd207256f2e5f7927ba5ebb81aad1345 https://hal-amu.archives-ouvertes.fr/hal-01692495/document |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....fd207256f2e5f7927ba5ebb81aad1345 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 09607412 1365313X |
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| DOI: | 10.1111/tpj.13498⟩ |