Epigallocatechin gallate inhibits leukotoxin release by Aggregatibacter actinomycetemcomitans by promoting association with the bacterial membrane
| العنوان: | Epigallocatechin gallate inhibits leukotoxin release by Aggregatibacter actinomycetemcomitans by promoting association with the bacterial membrane |
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| المؤلفون: | Nataliya Balashova, Angela C. Brown, Peter Giaquinto, En Hyung Chang, Joanne Huang |
| المصدر: | Mol Oral Microbiol |
| بيانات النشر: | Wiley, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | 0301 basic medicine, Microbiology (medical), Immunology, Exotoxins, Virulence, Epigallocatechin gallate, medicine.disease_cause, Aggregatibacter actinomycetemcomitans, Microbiology, Catechin, Article, Mice, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, medicine, Animals, Humans, Secretion, General Dentistry, Pathogen, Host cell membrane, Bacteria, biology, Toxin, Chemistry, food and beverages, 030206 dentistry, biology.organism_classification, Bacterial Outer Membrane, 030104 developmental biology, Antitoxin |
| الوصف: | The oral pathogen, Aggregatibacter actinomycetemcomitans, produces a number of virulence factors, including a leukotoxin (LtxA), which specifically kills human white blood cells, to provide a colonization advantage to the bacterium. Strains of A. actinomycetemcomitans that produce more LtxA have been more closely linked to disease, indicating that this toxin plays a key role in pathogenesis of the bacterium. Disruption of the activity of LtxA thus represents a promising approach to reducing the pathogenicity of the bacterium. Catechins are polyphenolic molecules derived from plants, which have shown potent antibacterial and antitoxin activities. We have previously shown that galloylated catechins are able to prevent LtxA delivery to host cells by altering the toxin’s secondary structure and preventing binding to cholesterol on the host cell membrane. Here, we have investigated how one particular galloylated catechin, epigallocatechin gallate (EGCg), affects A. actinomycetemcomitans growth and toxin secretion. Our results demonstrate that EGCg, at micromolar concentrations, inhibits A. actinomycetemcomitans growth, as has been reported for other bacterial species. At subinhibitory concentrations, EGCg promotes LtxA production, but the toxicity of the bacterial supernatant against human immune cells is reduced. The results of our biophysical studies indicate that this seemingly contradictory result is caused by an EGCg-mediated enhancement of LtxA affinity for the bacterial cell surface. Together, these results demonstrate the potential of EGCg in the treatment of virulent A. actinomycetemcomitans infections. |
| تدمد: | 2041-1014 2041-1006 |
| DOI: | 10.1111/omi.12275 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fcea1912c1a07f33a5eb5378b42bed16 https://doi.org/10.1111/omi.12275 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....fcea1912c1a07f33a5eb5378b42bed16 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20411014 20411006 |
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| DOI: | 10.1111/omi.12275 |