Msx2 plays an important role in BMP6-induced osteogenic differentiation of two mesenchymal cell lines: C3H10T1/2 and C2C12
| العنوان: | Msx2 plays an important role in BMP6-induced osteogenic differentiation of two mesenchymal cell lines: C3H10T1/2 and C2C12 |
|---|---|
| المؤلفون: | Ye Huang, He-ran Ma, Li Wen, Peng Xue, Na Huo, Jing Wang, Chuan Cai |
| المصدر: | Regenerative Therapy Regenerative Therapy, Vol 14, Iss, Pp 245-251 (2020) |
| بيانات النشر: | Japanese Society for Regenerative Medicine, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0301 basic medicine, Biomedical Engineering, SMAD, BMP6, Bone morphogenetic protein, Ad, adenovirus-transfection, Biomaterials, 03 medical and health sciences, Msx, Msh homeobox, 0302 clinical medicine, Osteogenesis, lcsh:QH573-671, BMP, Bone morphogenetic protein, Transcription factor, Adenovirus-transfection, lcsh:R5-920, lcsh:Cytology, Chemistry, Signaling pathway, Mesenchymal stem cell, siRNA, silencing RNA, Cell biology, Bone morphogenetic protein 6, 030104 developmental biology, Cell culture, Original Article, Signal transduction, lcsh:Medicine (General), C2C12, 030217 neurology & neurosurgery, Developmental Biology, Msx2 |
| الوصف: | Bone morphogenetic proteins (BMPs), have been shown to enhance the osteogenic differentiation of mesenchymal cells (MCs) and to promote bone formation. BMP6 is known to play an important role in the process of MCs towards osteogenic differentiation by virtue of their osteoinductive and cell type specific proliferative activity. However, the molecular mechanism relate to BMP6 osteoinductive activity is still unclear and continues to warrant further investigation. Msx2 is a member of the homeobox gene family of transcription factors and promotes calcification. Hence, we wondered if it might also play a role in BMP6-induced osteogenesis. In this study, two mouse mesenchymal cell lines were treated with BMP6, adenovirus-Msx2 (Ad-Msx2) or adenovirus-siMsx2 (Ad-siMsx2). Based on the results of mRNA and protein expression, it was indicated that BMP6 could enhance the expression of Msx2 and activate the phosphorylation of Smad 1/5/8, p38 and ERK1/2. Being transfected by Ad-Msx2, the BMP6-induced activation of phosphorylation was significantly promoted. On the contrary, two cell lines transfected by Ad-siMsx2 presented an inhibited expression of three phosphorylated proteins even after being induced by BMP6. The evaluation of ALP, OPN, OC and calcium deposits revealed the osteogenic results those were corresponding to the results of mRNA and protein. Taken together, these findings can be a novel viewpoint for the understanding of the mechanisms of BMP6-induced osteogenesis and provide therapeutic targets of bone defect. Highlights • BMP6 could promote the expression of Msx2 and enhance osteogenic differentiation of MCs. • Over-expressed Msx2 could reinforce the activation of Smad/MAPK signaling pathway which is triggered by BMP6. • Msx2 may play a key role in BMP6-induced osteogenesis. |
| اللغة: | English |
| تدمد: | 2352-3204 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fbb8c4263a4b3290b26d226593054933 http://europepmc.org/articles/PMC7232041 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....fbb8c4263a4b3290b26d226593054933 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 23523204 |
|---|