A Controversial Tumor Marker: Is SM22 a Proper Biomarker for Gastric Cancer Cells?
| العنوان: | A Controversial Tumor Marker: Is SM22 a Proper Biomarker for Gastric Cancer Cells? |
|---|---|
| المؤلفون: | Jianmin Shao, Maomao Sun, Ningzhi Xu, Na Li, Youyong Lu, Xianghong Li, Fuli Peng, Yu-Mei Liang, Siqi Liu, Rong Wang, Jun Zhang |
| المصدر: | Journal of Proteome Research. 6:3304-3312 |
| بيانات النشر: | American Chemical Society (ACS), 2007. |
| سنة النشر: | 2007 |
| مصطلحات موضوعية: | Muscle Proteins, Biology, medicine.disease_cause, Biochemistry, Stomach Neoplasms, Biomarkers, Tumor, medicine, Humans, Electrophoresis, Gel, Two-Dimensional, Cells, Cultured, Tumor marker, Tissue microarray, Microfilament Proteins, Cancer, Muscle, Smooth, General Chemistry, Fibroblasts, medicine.disease, Blot, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Immunology, Cancer cell, Cancer research, Biomarker (medicine), Carcinogenesis, Myofibroblast |
| الوصف: | SM22, a dominant protein in smooth muscle cells (SMCs), has been widely reported to be abnormally expressed in many solid tumors. However, the expression patterns of SM22 are not consistent in all tumors, not even in the same ones. Whether SM22 should be considered a tumor biomarker is still debated in different laboratories. Herein, we have carried out a systematical investigation to validate SM22 expression in the primary tissues of gastric cancer (GC). Of eight cases, seven samples were found in the elevated expression of SM22 proteins through proteomic analysis. The observation was further verified by the approaches of Western blotting and quantitative RT-PCR. Surprisingly, the results achieved from tissue microarray in 126 GC cases appeared contrary to the proteomic conclusion, in which the highly expressed SM22 was mainly found in smooth muscle layers, blood vessels, and myofibroblasts. This suggested that the increased abundance of SM22 in the cancerous regions was not caused by the presence of the GC cells. Furthermore, the expression of SM22 was measured in different GC cell lines and SMCs with Western blotting and quantitative RT-PCR. The results revealed that SM22 expression in SMCs was dramatically higher than that of the GC cells, which indicates that SM22 is unlikely to be a proper biomarker for GC. Instead, it can be considered a potential indicator for the abnormal developments of smooth muscles, blood vessels, or myofibroblasts triggered by tumorigenesis. |
| تدمد: | 1535-3907 1535-3893 |
| DOI: | 10.1021/pr0702363 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fabef75d020d548fa8447870d3bd3e65 https://doi.org/10.1021/pr0702363 |
| رقم الانضمام: | edsair.doi.dedup.....fabef75d020d548fa8447870d3bd3e65 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15353907 15353893 |
|---|---|
| DOI: | 10.1021/pr0702363 |