High-Throughput Microfluidic Real-Time PCR for the Detection of Multiple Microorganisms in Ixodid Cattle Ticks in Northeast Algeria
| العنوان: | High-Throughput Microfluidic Real-Time PCR for the Detection of Multiple Microorganisms in Ixodid Cattle Ticks in Northeast Algeria |
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| المؤلفون: | Clemence Galon, Naouelle Azzag, Ghania Boularias, Sara Moutailler, Henri-Jean Boulouis, Ladislav Šimo |
| المساهمون: | Higher National Veterinary School of Algiers (ENSV), Biologie moléculaire et immunologie parasitaires et fongiques (BIPAR), École nationale vétérinaire d'Alfort (ENVA)-Laboratoire de santé animale, sites de Maisons-Alfort et de Dozulé, Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Supported by the French Agency for Food, Environmental and Occupational Health and Safety (ANSES), Supported by UMR BIPAR, Maisons-Alfort, France., ANR-10-LABX-0062,IBEID,Integrative Biology of Emerging Infectious Diseases(2010), École nationale vétérinaire - Alfort (ENVA)-Laboratoire de santé animale, sites de Maisons-Alfort et de Dozulé |
| المصدر: | Pathogens Pathogens, MDPI, 2021, 10 (3), pp.1-14. ⟨10.3390/pathogens10030362⟩ Pathogens, Vol 10, Iss 362, p 362 (2021) Volume 10 Issue 3 |
| بيانات النشر: | HAL CCSD, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Microbiology (medical), Veterinary medicine, Hyalomma marginatum, Rhipicephalus sanguineus, 030231 tropical medicine, lcsh:Medicine, medicine.disease_cause, Article, ixodid ticks, 03 medical and health sciences, 0302 clinical medicine, co-infection, parasitic diseases, medicine, Immunology and Allergy, Molecular Biology, high-throughput microfluidic real time PCR, 030304 developmental biology, 2. Zero hunger, Rickettsia massiliae, Rhipicephalus annulatus, 0303 health sciences, General Immunology and Microbiology, biology, lcsh:R, biology.organism_classification, bacterial infections and mycoses, 3. Good health, Rhipicephalus, Infectious Diseases, Rickettsia helvetica, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, cattle, Algeria, Ixodes, tick-borne pathogens, Hyalomma |
| الوصف: | Ixodid ticks are hematophagous arthropods considered to be prominent ectoparasite vectors that have a negative impact on cattle, either through direct injury or via the transmission of several pathogens. In this study, we investigated the molecular infection rates of numerous tick-borne pathogens in ticks sampled on cattle from the Kabylia region, northeastern Algeria, using a high-throughput microfluidic real-time PCR system. A total of 235 ticks belonging to seven species of the genera Rhipicephalus, Hyalomma, and Ixodes were sampled on cattle and then screened for the presence of 36 different species of bacteria and protozoans. The most prevalent tick-borne microorganisms were Rickettsia spp. at 79.1%, followed by Francisella-like endosymbionts (62.9%), Theileria spp. (17.8%), Anaplasma spp. (14.4%), Bartonella spp. (6.8%), Borrelia spp. (6.8%), and Babesia spp. (2.5%). Among the 80.4% of ticks bearing microorganisms, 20%, 36.6%, 21.7%, and 2.1% were positive for one, two, three, and four different microorganisms, respectively. Rickettsia aeschlimannii was detected in Hyalomma marginatum, Hyalomma detritum, and Rhipicephalus bursa ticks. Rickettsia massiliae was found in Rhipicephalus sanguineus, and Rickettsiamonacensis and Rickettsia helvetica were detected in Ixodesricinus. Anaplasma marginale was found in all identified tick genera, but Anaplasma centrale was detected exclusively in Rhipicephalus spp. ticks. The DNA of Borrelia spp. and Bartonella spp. was identified in several tick species. Theileria orientalis was found in R. bursa, R. sanguineus, H. detritum, H. marginatum, and I. ricinus and Babesia bigemina was found in Rhipicephalus annulatus and R. sanguineus. Our study highlights the importance of tick-borne pathogens in cattle in Algeria. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 2076-0817 |
| DOI: | 10.3390/pathogens10030362⟩ |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f9a9a0cb41accfab2a5ed8f1de30506a https://hal.inrae.fr/hal-03315094/document |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....f9a9a0cb41accfab2a5ed8f1de30506a |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20760817 |
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| DOI: | 10.3390/pathogens10030362⟩ |