Metabolite mass spectrometry profiling of cacao genotypes reveals contrasting resistances to Ceratocystis cacaofunesta phytopathogen
| العنوان: | Metabolite mass spectrometry profiling of cacao genotypes reveals contrasting resistances to Ceratocystis cacaofunesta phytopathogen |
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| المؤلفون: | Ana Valéria Colnaghi Simionato, Marcos N. Eberlin, Edna Dora Martins Newman Luz, Fábio Neves dos Santos, Dilze Maria Argôlo Magalhães |
| المصدر: | ELECTROPHORESIS. 42:2519-2527 |
| بيانات النشر: | Wiley, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Genotype, Metabolite, Clinical Biochemistry, Fungus, Ceratocystis, Biochemistry, Analytical Chemistry, chemistry.chemical_compound, Metabolomics, Tandem Mass Spectrometry, medicine, Theobromine, Disease Resistance, Plant Diseases, Cacao, Methyl jasmonate, biology, Jasmonic acid, Ceratocystis cacaofunesta, biology.organism_classification, chemistry, Chromatography, Liquid, medicine.drug |
| الوصف: | Ceratocystis wilt is a lethal disease of cacao, and the search for resistant genotypes may provide the best way to deal with the disease. Resistance or susceptibility behavior of some cacao genotypes when infected by Ceratocystis cacaofunesta is not yet understood. Herein, we report an LC-MS metabolomic screening analysis based on high-resolution MS to obtain comprehensive metabolic profile associated with multivariate data analysis of PLS-DA, which was effective to classify CCN-51 and TSH-1188 as resistant genotypes to C. cacaofunesta fungus, while CEPEC2002 was classified as a susceptible one. Using reversed-phase LC method, electrospray interface, and high-resolution tandem MS by the quadrupole-TOF analyzer, the typical profiles of metabolites, such as phenylpropanoids, flavonoids, lipids, alkaloids, and amino acids, were obtained. Untargeted metabolite profiles were used to construct discriminant analysis by partial least squares (PLS-DA)-derived loading plots, which placed the cacao genotypes into two major clusters related to susceptible or resistant groups. Linolenic, linoleic, oleic, stearic, arachidonic, and asiatic acids were annotated metabolites of infected, susceptible, and resistant genotypes, while methyl jasmonate, jasmonic acid, hydroxylated jasmonic acid, caffeine, and theobromine were annotated as constituents of the resistant genotypes. Trends of these typical metabolites levels revealed that CCN51 is susceptible, CEPEC2002 is moderately susceptible, and TSH1188 is resistant to C. cacaofunesta. Therefore, profiles of major metabolites as screened by LC-MS offer an efficient tool to reveal the level of resistance of cacao genotypes to C. cacaofunesta present in any farm around the world. |
| تدمد: | 1522-2683 0173-0835 |
| DOI: | 10.1002/elps.202100097 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f984e4a02c13e5dafcf2f755d9a467a1 https://doi.org/10.1002/elps.202100097 |
| Rights: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....f984e4a02c13e5dafcf2f755d9a467a1 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15222683 01730835 |
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| DOI: | 10.1002/elps.202100097 |