Intranasal Vaccine Using P10 Peptide Complexed within Chitosan Polymeric Nanoparticles as Experimental Therapy for Paracoccidioidomycosis in Murine Model
| العنوان: | Intranasal Vaccine Using P10 Peptide Complexed within Chitosan Polymeric Nanoparticles as Experimental Therapy for Paracoccidioidomycosis in Murine Model |
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| المؤلفون: | Andre Correa Amaral, Leandro Buffoni Roque da Silva, Luiz R. Travassos, Lucas Dos Santos Dias, Francenya Kelley Lopes da Silva, Ana Camila Oliveira Souza, Carlos Pelleschi Taborda, Marcelo Valdemir de Araújo, Samuel Rodrigues Dos Santos Junior |
| المصدر: | Journal of Fungi Volume 6 Issue 3 Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP Journal of Fungi, Vol 6, Iss 160, p 160 (2020) |
| بيانات النشر: | MDPI, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Microbiology (medical), 030231 tropical medicine, Peptide, Plant Science, Paracoccidioides, Article, Microbiology, Chitosan, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Immune system, GLICOPROTEÍNAS, paracoccidioidomycosis, Zeta potential, medicine, Viability assay, P10 peptide, lcsh:QH301-705.5, Ecology, Evolution, Behavior and Systematics, 030304 developmental biology, Paracoccidioides brasiliensis, chemistry.chemical_classification, 0303 health sciences, biology, Chemistry, Paracoccidioidomycosis, biology.organism_classification, medicine.disease, antifungal therapy, polymeric nanoparticles, lcsh:Biology (General), nanovaccine |
| الوصف: | Paracoccidioidomycosis (PCM) is a granulomatous fungal disease caused by the dimorphic fungal species of Paracoccidioides, which mainly affects the lungs. Modern strategies for the treatment and/or prevention of PCM are based on a Th1-type immune response, which is important for controlling the disease. One of the most studied candidates for a vaccine is the P10 peptide, derived from the 43 kDa glycoprotein of Paracoccidioides brasiliensis. In order to improve its immune modulatory effect, the P10 peptide was associated with a chitosan-conjugated nanoparticle. The nanoparticles presented 220 nm medium size, poly dispersion index (PDI) below 0.5, zeta potential of +20 mV and encapsulation efficiency around 90%. The nanoparticles&rsquo non-toxicity was verified by hemolytic test and cell viability using murine macrophages. The nanoparticles were stable and presented physicochemical characteristics desirable for biological applications, reducing the fungal load and the usual standard concentration of the peptide from 4 to 20 times. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 2309-608X |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f8e4330872f0464758b54a5a8ae8fd83 http://europepmc.org/articles/PMC7560165 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....f8e4330872f0464758b54a5a8ae8fd83 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 2309608X |
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