Visualization of interactions between siderophore transporters and the energizing protein TonB by native PAGE
| العنوان: | Visualization of interactions between siderophore transporters and the energizing protein TonB by native PAGE |
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| المؤلفون: | Hendrik Adams, Hervé Celia, Franc Pattus, Souhaila Choul-Li |
| المساهمون: | Institut Gilbert-Laustriat : Biomolécules, Biotechnologie, Innovation Thérapeutique, Université Louis Pasteur - Strasbourg I-Centre National de la Recherche Scientifique (CNRS) |
| المصدر: | Electrophoresis Electrophoresis, Wiley-VCH Verlag, 2008, 29 (6), pp.1333-1338. ⟨10.1002/elps.200700612⟩ |
| سنة النشر: | 2008 |
| مصطلحات موضوعية: | Siderophore, biology, Clinical Biochemistry, Membrane Proteins, Siderophores, Transporter, Gel electrophoresis of proteins, biology.organism_classification, Biochemistry, Analytical Chemistry, Protein–protein interaction, Membrane protein, Bacterial Proteins, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, Electrophoresis, Polyacrylamide Gel, Bacterial outer membrane, Bacteria, Organism, Bacterial Outer Membrane Proteins |
| الوصف: | Horizontal nondenaturing electrophoresis of proteins in polyacrylamide gels was used to observe specific interactions between membrane proteins. The method was particularly well suited for solubilized transporters of the outer membrane of Gram-negative bacteria, and allowed specific complexes of transporter and the inner-membrane protein TonB to be isolated. We have used this method to investigate the interactions between four different outer-membrane transporters, and the TonB proteins from two different organisms. The results show that a stable complex can be isolated on gels for all the proteins studied, but can depend in some cases of the detergent used for solubilization. Furthermore, we observe cross-species interaction as TonB from a given organism can interact with transporters from another organism. |
| تدمد: | 0173-0835 1522-2683 |
| DOI: | 10.1002/elps.200700612⟩ |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f859525b85e6f4f9282387daee8f71b2 https://pubmed.ncbi.nlm.nih.gov/18288671 |
| Rights: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....f859525b85e6f4f9282387daee8f71b2 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 01730835 15222683 |
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| DOI: | 10.1002/elps.200700612⟩ |