Nanostructuring Lipophilic Dyes in Water Using Stable Vesicles, Quatsomes, as Scaffolds and Their Use as Probes for Bioimaging
| العنوان: | Nanostructuring Lipophilic Dyes in Water Using Stable Vesicles, Quatsomes, as Scaffolds and Their Use as Probes for Bioimaging |
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| المؤلفون: | Cristina Sissa, Siarhei Kurhuzenkau, David J. Hagan, Natalia Feiner, Jordi Faraudo, Lorenzo Albertazzi, Sílvia Illa-Tuset, Eric W. Van Stryland, Anna Painelli, Antonio Ardizzone, Nora Ventosa, Jaume Veciana, Mykhailo V. Bondar |
| المساهمون: | Ministerio de Economía y Competitividad (España), Generalitat de Catalunya, Centro de Investigación Biomédica en Red Bioingeniería, Biomateriales y Nanomedicina (España), AXA Research Fund, US Army Research Laboratory, European Commission, Molecular Biosensing for Med. Diagnostics |
| المصدر: | Digital.CSIC. Repositorio Institucional del CSIC instname Small : Nano Micro, 14(16):1703851. Wiley-VCH Verlag |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | STORM, Nanoparticle, Nanotechnology, 02 engineering and technology, Fluorescent nanoparticles, Molecular dynamics, Molecular Dynamics Simulation, 010402 general chemistry, 01 natural sciences, Nanomaterials, Biomaterials, Perchlorate, chemistry.chemical_compound, General Materials Science, Vesicles, Carbocyanines, Dyes, Chemistry, Vesicle, Water, General Chemistry, 021001 nanoscience & nanotechnology, Fluorescence, 3. Good health, 0104 chemical sciences, Nanostructures, Membrane, Water/chemistry, Nanoparticles, Nanoparticles/chemistry, 0210 nano-technology, Biological imaging, Nanostructures/chemistry, Biotechnology |
| الوصف: | A new kind of fluorescent organic nanoparticles (FONs) is obtained using quatsomes (QSs), a family of nanovesicles proposed as scaffolds for the nanostructuration of commercial lipophilic carbocyanines (1,1′‐dioctadecyl‐3,3,3′,3′‐tetramethyl‐indocarbocyanine perchlorate (DiI), 1,1′‐dioctadecyl‐3,3,3′,3′‐tetramethyl‐indodicarbocyanine perchlorate (DiD), and 1,1′‐dioctadecyl‐3,3,3′,3′‐tetramethyl‐indotricarbocyanine iodide (DiR)) in aqueous media. The obtained FONs, prepared by a CO2‐based technology, show excellent colloidal‐ and photostability, outperforming other nanoformulations of the dyes, and improve the optical properties of the fluorophores in water. Molecular dynamics simulations provide an atomistic picture of the disposition of the dyes within the membrane. The potential of QSs for biological imaging is demonstrated by performing superresolution microscopy of the DiI‐loaded vesicles in vitro and in cells. Therefore, fluorescent QSs constitute an appealing nanomaterial for bioimaging applications. This work was financially supported by MINECO/FEDER, Spain (Grant Nos. CTQ2013‐40480‐R, SAF2016‐7524‐R, and MAT 2016‐80826‐R), by AGAUR, Generalitat de Catalunya (Grant No. 2014‐SGR‐17), and through the CERCA program, the Networking Research Center on Bioengineering, Biomaterials, and Nanomedicine (CIBER‐BBN) and the Spanish Ministry of Economy and Competitiveness, through the “Severo Ochoa” Programme for Centres of Excellence in R&D (SEV‐2015‐0496, Grant FLOWERS, and SEV‐2014‐0425). L.A. thanks the financial support of AXA Research Fund and D.H. and E.V.S. the Army Research Laboratory (Grant No. W911NF‐15‐2‐0090). The research leading to these results received funding from the People Programme (Marie Curie Actions) of the European Union's Seventh Framework ProgrammeFP7/2007–2013 under REA grant Agreement No. 607721 (Nano2Fun) A.A. and S.I.‐T. are enrolled in the Materials Science Ph.D. program of UAB. A.A. and S.I.‐T. gratefully acknowledge help of Dr. M. Masino in photostability measurements. |
| تدمد: | 1613-6829 1613-6810 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f5d6ef673839539004cbe707b9e763a5 https://pubmed.ncbi.nlm.nih.gov/29573545 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....f5d6ef673839539004cbe707b9e763a5 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 16136829 16136810 |
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