In Operando Observation of Neuropeptide Capture and Release on Graphene Field-Effect Transistor Biosensors with Picomolar Sensitivity
| العنوان: | In Operando Observation of Neuropeptide Capture and Release on Graphene Field-Effect Transistor Biosensors with Picomolar Sensitivity |
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| المؤلفون: | Cameron M. Crasto, Joshua A. Hagen, Rajesh R. Naik, Oksana M. Pavlyuk, Joseph M. Slocik, Nancy Kelley-Loughnane, Ming-Siao Hsiao, Lawrence F. Drummy, Yen Ngo, Steve S. Kim, Li Xing, Rhett L. Martineau, Ahmad E. Islam, Cheri M. Hampton, Madhavi P. Kadakia |
| المصدر: | ACS Applied Materials & Interfaces. 11:13927-13934 |
| بيانات النشر: | American Chemical Society (ACS), 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Analyte, Materials science, Transistors, Electronic, Cryo-electron microscopy, Nanotechnology, Biosensing Techniques, 02 engineering and technology, 010402 general chemistry, 01 natural sciences, law.invention, Responsivity, Peptide Library, law, Humans, Molecule, Neuropeptide Y, General Materials Science, Sweat, Graphene, Cryoelectron Microscopy, Transistor, 021001 nanoscience & nanotechnology, 0104 chemical sciences, Coupling (electronics), ROC Curve, Area Under Curve, Graphite, 0210 nano-technology, Biosensor, Protein Binding |
| الوصف: | Transmission electron microscopy (TEM) is being pushed to new capabilities which enable studies on systems that were previously out of reach. Among recent innovations, TEM through liquid cells (LC-TEM) enables in operando observation of biological phenomena. This work applies LC-TEM to the study of biological components as they interact on an abiotic surface. Specifically, analytes or target molecules like neuropeptide Y (NPY) are observed in operando on functional graphene field-effect transistor (GFET) biosensors. Biological recognition elements (BREs) identified using biopanning with affinity to NPY are used to functionalize graphene to obtain selectivity. On working devices capable of achieving picomolar responsivity to neuropeptide Y, LC-TEM reveals translational motion, stochastic positional fluctuations due to constrained Brownian motion, and rotational dynamics of captured analyte. Coupling these observations with the electrical responses of the GFET biosensors in response to analyte capture and/or release will potentially enable new insights leading to more advanced and capable biosensor designs. |
| تدمد: | 1944-8252 1944-8244 |
| DOI: | 10.1021/acsami.8b20498 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f57e88e31ea9be6e02cbe74086bc835d https://doi.org/10.1021/acsami.8b20498 |
| Rights: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....f57e88e31ea9be6e02cbe74086bc835d |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19448252 19448244 |
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| DOI: | 10.1021/acsami.8b20498 |