sym 13—A Gene Conditioning Ineffective Nodulation in Pisum sativum
| العنوان: | sym 13—A Gene Conditioning Ineffective Nodulation in Pisum sativum |
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| المؤلفون: | Carol A. Smith, Ann M. Hirsch, Barbara E. Kneen, Thomas A. LaRue, Norman F. Weeden |
| المصدر: | Plant Physiology. 94:899-905 |
| بيانات النشر: | Oxford University Press (OUP), 1990. |
| سنة النشر: | 1990 |
| مصطلحات موضوعية: | Genetics, Ethyl methanesulfonate, Physiology, Mutant, Locus (genetics), Plant Science, Biology, medicine.disease_cause, biology.organism_classification, Rhizobium leguminosarum, Pisum, chemistry.chemical_compound, Sativum, chemistry, Gene mapping, medicine, Microbe-Plant Interactions, Gene |
| الوصف: | Treatment of Pisum sativum (L.) cv. `Sparkle9 with ethyl methanesulfonic acid (EMS) produced a stable mutant, E135F, which forms small, white, ineffective nodules. These nodules exhibit histological zonation typical of an indeterminant nodule, e.g. meristematic, early symbiotic, late symbiotic, and senescent zones. Compared with the nitrogen fixing nodules of the parent, the zones are smaller and the nodules senesce prematurely. Bacteroids in E135F are less elongated and less differentiated than those in `Sparkle.9 The E135F mutant forms ineffective nodules when inoculated with nine different effective strains of Rhizobium leguminosarum and also when grown in a soil containing effective strains. The ineffective phenotype of E135F is under monogenic recessive control; the gene is designated sym 13. sym 13 was located on chromosome 2 by linkage with genes for shikimic dehydrogenase and esterase-2. The original selection E135F carried another mutation in heterozygous form at a separate locus, yielding some homozygous recessive nonnodulating progeny, E135N, in later generations. This indicates that EMS treatments may cause mutations at more than one sym gene. The gene conditioning non-nodulation in E135N was designated sym 14. It mapped to a locus on a different part of chromosome 2 by linkage to the gene for fumarase. The data demonstrate that sym genes are not necessarily closely linked. |
| تدمد: | 1532-2548 0032-0889 |
| DOI: | 10.1104/pp.94.3.899 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f4e8624bc143a4158e12b2989ad0333d https://doi.org/10.1104/pp.94.3.899 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....f4e8624bc143a4158e12b2989ad0333d |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15322548 00320889 |
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| DOI: | 10.1104/pp.94.3.899 |