Steady state pig dendritic cells migrating in skin draining pseudo-afferent lymph are semi-mature
| العنوان: | Steady state pig dendritic cells migrating in skin draining pseudo-afferent lymph are semi-mature |
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| المؤلفون: | Florian Marquet, Florentina Pascale, Michel Bonneau, Chantal Kang, Nicolas Bertho, Isabelle Schwartz-Cornil |
| المساهمون: | Unité de recherche Virologie et Immunologie Moléculaires (VIM (UR 0892)), Institut National de la Recherche Agronomique (INRA), UE 1298 Unité Commune d'Expérimentation Animale |
| المصدر: | Veterinary Immunology and Immunopathology Veterinary Immunology and Immunopathology, Elsevier, 2011, 144 (3-4), pp.430-436. ⟨10.1016/j.vetimm.2011.09.007⟩ |
| بيانات النشر: | Elsevier BV, 2011. |
| سنة النشر: | 2011 |
| مصطلحات موضوعية: | CD4-Positive T-Lymphocytes, Lipopolysaccharide, [SDV]Life Sciences [q-bio], T cell, Immunology, Antigen presentation, Stimulation, Biology, migration, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Antigen, Cell Movement, veterinary sciences, medicine, Animals, [INFO]Computer Science [cs], Pathogen, Skin, 030304 developmental biology, 0303 health sciences, tolerance, General Veterinary, maturation, swine, antigen presentation, Phenotype, medicine.anatomical_structure, chemistry, Langerhans Cells, Lymph, Steady state (chemistry), 030215 immunology |
| الوصف: | International audience; Dendritic cells (DC) in peripheral tissues are considered as immature cells that mature and migrate towards lymph nodes upon stimulation with pathogens. This commonly accepted paradigm is challenged by the fact that tolerance to peripheral self antigen is controlled by mature DC and that DC collected from afferent lymph draining different tissues from several species, in the absence of pathogen signaling, were inconsistently found to be either at a mature or semi-mature state. In order to better define the maturation state of DC that migrate in lymph in absence of pathogen stimulation, we compared skin lymph DC to resident and LPS (lipopolysaccharide)-activated skin DC thanks to the establishment of a mini-pig model of lymph duct cannulation. Based on their co-stimulatory molecules expression and endocytotic capacities, pig lymph skin DC were found at an intermediate state of maturation between resident and LPS-activated skin DC and were fully capable of allogeneic T cell stimulation. Furthermore, lymph skin DC could be further matured by LPS or influenza stimulation. Thus, using the pig skin model which is relevant to human, we show that skin-derived DC constantly migrate at an intermediate state of maturation that can be further enhanced upon appropriate stimulation. |
| تدمد: | 0165-2427 |
| DOI: | 10.1016/j.vetimm.2011.09.007 |
| DOI: | 10.1016/j.vetimm.2011.09.007⟩ |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f344a3eaf0049566b7c7c53b745f182e https://doi.org/10.1016/j.vetimm.2011.09.007 |
| Rights: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....f344a3eaf0049566b7c7c53b745f182e |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 01652427 |
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| DOI: | 10.1016/j.vetimm.2011.09.007 |