DNA damage foci formation and decline in two-dimensional monolayers and in three-dimensional human vessel models: Differential effects according to radiation quality
| العنوان: | DNA damage foci formation and decline in two-dimensional monolayers and in three-dimensional human vessel models: Differential effects according to radiation quality |
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| المؤلفون: | Charles R. Geard, Alan W. Bigelow, Peter Grabham |
| المصدر: | International Journal of Radiation Biology. 88:493-500 |
| بيانات النشر: | Informa UK Limited, 2012. |
| سنة النشر: | 2012 |
| مصطلحات موضوعية: | Models, Anatomic, DNA damage, Kinetics, Immunocytochemistry, Linear energy transfer, Radiation, Umbilical vein, chemistry.chemical_compound, Imaging, Three-Dimensional, Human Umbilical Vein Endothelial Cells, Humans, Linear Energy Transfer, Radiology, Nuclear Medicine and imaging, Radiological and Ultrasound Technology, Chemistry, Intracellular Signaling Peptides and Proteins, Models, Cardiovascular, Gamma ray, Crystallography, Gamma Rays, Biophysics, Blood Vessels, Protons, Tumor Suppressor p53-Binding Protein 1, Relative Biological Effectiveness, DNA, DNA Damage |
| الوصف: | To analyze the effect of different radiation qualities on the kinetics of p53 Binding Protein 1 (53BP1) formation and decline in human three-dimensional (3-D) vessel models.Two-dimensional (2-D) and 3-D cultures of human umbilical vein cells were exposed to 80 cGy of Gamma radiation and high-energy protons and Fe ions. 53BP1 antibodies were used for foci visualization via immunocytochemistry. Computer analysis was used to determine the number and the size of foci up to 48 hours after irradiation.DNA foci kinetics in 2-D and 3-D human vessel cultures show that foci formation and removal were the same in each type of culture. After 48 h, the number of foci induced by high-energy protons and gamma rays reduced to almost control levels while high linear energy transfer (LET) Fe particles produced more persistent damage.The kinetics of radiation-induced 53BP1 foci in 3-D vessel models is essentially the same as in 2-D monolayers. Since the basal level of spontaneous foci is low in these differentiated non-proliferating cultures, the persistence of radiation-induced 53BP1 foci is detected longer than previously noted. Furthermore, analysis of foci sizes revealed that abnormal radiation-induced foci can persist even when foci frequencies are close to basal levels. The detection of these latent abnormalities could be useful for a more sensitive dosimetry. |
| تدمد: | 1362-3095 0955-3002 |
| DOI: | 10.3109/09553002.2012.679382 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f30be3675cc779f55298d8abf3e101eb https://doi.org/10.3109/09553002.2012.679382 |
| رقم الانضمام: | edsair.doi.dedup.....f30be3675cc779f55298d8abf3e101eb |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 13623095 09553002 |
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| DOI: | 10.3109/09553002.2012.679382 |