التفاصيل البيبلوغرافية
| العنوان: |
Eventual limits of the current EU official method for evaluating milk adulteration of water buffalo dairy products and potential proteomic solutions |
| المؤلفون: |
Gabriella Pinto, Simonetta Caira, Francesco Addeo, Lina Chianese, Andrea Scaloni, Maria Grazia Calabrese, Maria Adalgisa Nicolai, Sergio Lilla |
| المساهمون: |
Caira, S, Nicolai, MARIA ADALGISA, Lilla, S, Calabrese, Mg, Pinto, Gabriella, Scaloni, A, Chianese, Lina, Addeo, Francesco |
| المصدر: |
Food chemistry 230 (2017): 482–490. doi:10.1016/j.foodchem.2017.03.055
info:cnr-pdr/source/autori:Simonetta Caira, Maria Adalgisa Nicolai, Sergio Lilla, Maria Grazia Calabrese, Gabriella Pinto, Andrea Scaloni, Lina Chianese, Francesco Addeo/titolo:Eventual limits of the current EU official method for evaluating milk adulteration of water buffalo dairy products and potential proteomic solutions/doi:10.1016%2Fj.foodchem.2017.03.055/rivista:Food chemistry/anno:2017/pagina_da:482/pagina_a:490/intervallo_pagine:482–490/volume:230 |
| بيانات النشر: |
Applied Science Publishers., London, Regno Unito, 2017. |
| سنة النشر: |
2017 |
| مصطلحات موضوعية: |
0301 basic medicine, Proteomics, Resolution (mass spectrometry), Buffaloes, Hydroxyapatite column, 01 natural sciences, Analytical Chemistry, 03 medical and health sciences, Cheese, Animals, Peptide markers, Detection limit, g2- and g3-casein Peptide markers Milk Cheese Proteomics, Chromatography, Chemistry, Isoelectric focusing, 010401 analytical chemistry, Caseins, General Medicine, Beta2- and Beta3-casein, 0104 chemical sciences, 030104 developmental biology, Milk, Water buffalo, Cattle, Food Science |
| الوصف: |
The European reference method (ERM) recognises the fraudulent addition of bovine (B) milk in water buffalo (WB) milk/dairy products based on concomitant isoelectric focusing (IEF) detection of B Beta2- and Beta3-CN fragments after corresponding plasminolysis. We here used proteomics to characterise false positive results occurring in the ERM as being due to WB Beta-CN(f100-209), which is also formed after plasminolysis of genuine WB milk/dairy products and comigrates in IEF with B Beta2-CN. These ERM limitations were overcome by a dedicated proteomic procedure based on loading of B/WB milk/cheese CN extracts on a hydroxyapatite column, in situ trypsinolysis and elution of B Beta-CN(f1-25)4P and WB Beta-CN(f1-28)4P proteotypic peptides. Based on their similar ionisation properties and resolution in MALDI-TOF-MS, these phosphopeptides were identified as suitable markers for detection of B material in WB milk/dairy products to a detection limit of 0.8% v/v. This proteomic procedure is here proposed as integrative/alternative to the ERM. |
| اللغة: |
English |
| DOI: |
10.1016/j.foodchem.2017.03.055 |
| URL الوصول: |
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f1ca2045af1dad83fb64789182c14de3 |
| Rights: |
RESTRICTED |
| رقم الانضمام: |
edsair.doi.dedup.....f1ca2045af1dad83fb64789182c14de3 |
| قاعدة البيانات: |
OpenAIRE |