LncRNA‑AWPPH activates TGF‑β1 in colorectal adenocarcinoma

التفاصيل البيبلوغرافية
العنوان: LncRNA‑AWPPH activates TGF‑β1 in colorectal adenocarcinoma
المؤلفون: Jianjun Xin, Chengcong Liu, Cheng Yang, Bo Han
المصدر: Oncology Letters
بيانات النشر: Spandidos Publications, 2019.
سنة النشر: 2019
مصطلحات موضوعية: lncRNA-AWPPH, 0301 basic medicine, Cancer Research, transforming growth factor β1, Oncogene, Cell growth, business.industry, Cell, Cancer, Articles, Cell cycle, medicine.disease, Molecular medicine, colorectal adenocarcinoma, 03 medical and health sciences, 030104 developmental biology, 0302 clinical medicine, medicine.anatomical_structure, Oncology, 030220 oncology & carcinogenesis, medicine, Cancer research, Gene silencing, Viability assay, business
الوصف: Long non-coding RNA associated with poor prognosis of hepatocellular carcinoma (lncRNA-AWPPH) is a newly discovered lncRNA that has important functions in the pathogenesis of several malignancies. However, its role in the development of colorectal adenocarcinoma is unknown. The current study therefore investigated the function of AWPPH in colorectal adenocarcinoma. A total of 86 patients with colorectal adenocarcinoma and 56 healthy controls were included. Tumor tissues and adjacent healthy tissues were collected from patients with colorectal adenocarcinoma, and blood was collected from both patients and healthy controls. Expression of AWPPH in tissues and blood was detected by the reverse transcription-quantitative polymerase chain reaction. Receiver operating characteristic curve analysis was used to evaluate the diagnostic value of serum AWPPH for colorectal adenocarcinoma. All patients were followed up for 5 years, and survival curve analysis was performed to investigate the association between serum level of AWPPH and patients' survival. The effects of AWPPH overexpression and silencing in colorectal adenocarcinoma cell lines were investigated. Effects on cell proliferation and viability were detected by the cell counting kit-8 and MTT assays, respectively. Effects on transforming growth factor β1 (TGF-β1) expression were determined by western blotting. AWPPH was significantly upregulated in tumor tissues compared with adjacent healthy tissues. AWPPH expression levels in blood increased in patients with colorectal adenocarcinoma compared with healthy controls, suggesting that AWPPH may be a sensitive and accurate diagnostic and prognostic biomarker for colorectal adenocarcinoma. AWPPH overexpression in colorectal adenocarcinoma cell lines promoted cell proliferation and increased cell viability, while AWPPH silencing resulted in opposite effects. AWPPH overexpression promoted and silencing inhibited TGF-β1 expression. Therefore, lncRNA-AWPPH promoted colorectal adenocarcinoma by promoting tumor growth, increasing tumor cell viability and activating the TGF-β1 signaling.
تدمد: 1792-1082
1792-1074
DOI: 10.3892/ol.2019.10794
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f19f6d84d316eaee8ba24938054930aa
https://doi.org/10.3892/ol.2019.10794
Rights: OPEN
رقم الانضمام: edsair.doi.dedup.....f19f6d84d316eaee8ba24938054930aa
قاعدة البيانات: OpenAIRE
الوصف
تدمد:17921082
17921074
DOI:10.3892/ol.2019.10794