Effect of transmembrane pressure on Factor VIII yield in ATF perfusion culture for the production of recombinant human Factor VIII co-expressed with von Willebrand factor
| العنوان: | Effect of transmembrane pressure on Factor VIII yield in ATF perfusion culture for the production of recombinant human Factor VIII co-expressed with von Willebrand factor |
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| المؤلفون: | Beom-Soo Park, Byung-Gee Kim, Sora An, Kyu-Heum Na, Seungchul Kim, Hyunki Kim |
| المصدر: | Cytotechnology. 68:1687-1696 |
| بيانات النشر: | Springer Science and Business Media LLC, 2015. |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | 0301 basic medicine, congenital, hereditary, and neonatal diseases and abnormalities, animal diseases, Recombinant human factor VIII, Clinical Biochemistry, Cell, Biomedical Engineering, Bioengineering, Transmembrane pressure, 03 medical and health sciences, Perfusion Culture, Von Willebrand factor, hemic and lymphatic diseases, medicine, Bioreactor, biology, Chemistry, Cell Biology, 030104 developmental biology, medicine.anatomical_structure, Yield (chemistry), Biophysics, biology.protein, Original Article, Perfusion, Biotechnology |
| الوصف: | In this study, we evaluated three cell retention devices, an alternating tangential flow (ATF) system, a spin-filter, and a Centritech Lab III centrifuge, for the production of recombinant human Factor VIII co-expressed with von Willebrand factor. From the results, it was found that the FVIII activity in bioreactor was significantly higher in the ATF perfusion culture than two other perfusion cultures. Moreover, the FVIII activity yield was unexpectedly low in the ATF perfusion culture. We have, therefore, studied the reasons for this low FVIII activity yield. It was revealed that the inactivation and the surface adsorption of FVIII onto the harvest bag were not the main reasons for the low yield in the ATF perfusion culture. The FVIII activity yield was not increased by the use of a hollow fiber filter with 0.5 μm pore size instead of 0.2 μm pore size. Additionally, the retention of FVIII molecules by the hollow fiber filter was a dominant factor in the low FVIII activity yield in the ATF perfusion culture. We demonstrated that FVIII yield was significantly improved by controlling transmembrane pressure (TMP) across the hollow fiber filter membrane. Taken together, these results suggest that TMP control could be an efficient method for the enhancement of FVIII yield in an ATF perfusion culture. |
| تدمد: | 1573-0778 0920-9069 |
| DOI: | 10.1007/s10616-015-9918-1 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f0d5ed63e98db4c1aa4f868d9bbed999 https://doi.org/10.1007/s10616-015-9918-1 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....f0d5ed63e98db4c1aa4f868d9bbed999 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15730778 09209069 |
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| DOI: | 10.1007/s10616-015-9918-1 |