MCT1 and MCT4 Kinetic of mRNA Expression in Different Tissues After Aerobic Exercise at Maximal Lactate Steady State Workload
| العنوان: | MCT1 and MCT4 Kinetic of mRNA Expression in Different Tissues After Aerobic Exercise at Maximal Lactate Steady State Workload |
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| المؤلفون: | G. G. De Araujo, F. de Barros Manchado-Gobatto, Marcelo Papoti, Luis Felipe Milano Teixeira, I. G. M. dos Reis, R. Verlengia, Claudio Alexandre Gobatto, Silvana Bordin, Cláudia Regina Cavaglieri, Luciana C. Caperuto |
| المصدر: | ResearcherID Scopus-Elsevier Europe PubMed Central |
| بيانات النشر: | Institute of Physiology of the Czech Academy of Sciences, 2015. |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | Male, Monocarboxylic Acid Transporters, medicine.medical_specialty, Anaerobic Threshold, Physiology, Physical Exertion, Biology, Mice, Physical Conditioning, Animal, Internal medicine, microRNA, Gene expression, medicine, Animals, Aerobic exercise, Tissue Distribution, Muscle, Skeletal, Swimming, Soleus muscle, Symporters, Myocardium, Skeletal muscle, General Medicine, Anatomy, Mice, Inbred C57BL, MicroRNAs, Endocrinology, medicine.anatomical_structure, Liver, Organ Specificity, Symporter, Steady state (chemistry), Anaerobic exercise |
| الوصف: | We evaluate the mRNA expression of monocarboxylate transporters 1 and 4 (MCT1 and MCT4) in skeletal muscle (soleus, red and white gastrocnemius), heart and liver tissues in mice submitted to a single bout of swimming exercise at the maximal lactate steady state workload (MLSSw). After 72 h of MLSS test, the animals were submitted to a swimming exercise session for 25 min at individual MLSSw. Tissues and muscle samples were obtained at rest (control, n=5), immediately (n=5), 5 h (n=5) and 10 h (n=5) after exercise for determination of the MCT1 and MCT4 mRNA expression (RT-PCR). The MCT1 mRNA expression in liver increased after 10 h in relation to the control, immediate and 5 h groups, but the MCT4 remained unchanged. The MCT1 mRNA expression in heart increased by 31 % after 10 h when compared to immediate, but no differences were observed in relation to the control group. No significant differences were observed for red gastrocnemius in MCT1 and MCT4 mRNA expression. However, white gastrocnemius increased MCT1 mRNA expression immediately when compared to rest, 5 and 10 h test groups. In soleus muscle, the MCT1 mRNA expression increased immediately, 5 and 10 h after exercise when compared to the control. In relation to MCT4 mRNA expression, the soleus increased immediately and 10 h after acute exercise when compared to the control group. The soleus, liver and heart were the main tissues that showed improved the MCT1 mRNA expression, indicating its important role in controlling MLSS concentration in mice. |
| تدمد: | 1802-9973 0862-8408 |
| DOI: | 10.33549/physiolres.932695 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f033ba94e22c5fde935d4f0042d9c282 https://doi.org/10.33549/physiolres.932695 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....f033ba94e22c5fde935d4f0042d9c282 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 18029973 08628408 |
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| DOI: | 10.33549/physiolres.932695 |