A New Approach for Prostate Cancer Diagnosis by miRNA Profiling of Prostate-Derived Plasma Small Extracellular Vesicles
| العنوان: | A New Approach for Prostate Cancer Diagnosis by miRNA Profiling of Prostate-Derived Plasma Small Extracellular Vesicles |
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| المؤلفون: | Margarita Knyazeva, Anastasia Malek, Sergey Reva, Maria Slyusarenko, Tsyrlina Ev, Nadezhda Nikiforova, Elena Sidina, Sergey Novikov, Lidia Zabegina, Inga Nazarova |
| المصدر: | Cells Volume 10 Issue 9 Cells, Vol 10, Iss 2372, p 2372 (2021) |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Male, SEVs, QH301-705.5, Aptamer, viruses, Population, small extracellular vesicles, urologic and male genital diseases, Article, Flow cytometry, Prostate cancer, Extracellular Vesicles, Prostate, PSMA(+)SEVs, microRNA, medicine, Biomarkers, Tumor, diagnostics, PSMA, Humans, Biology (General), education, Aged, miRNA, education.field_of_study, biology, medicine.diagnostic_test, Chemistry, Cancer, virus diseases, Prostatic Neoplasms, General Medicine, respiratory system, Aptamers, Nucleotide, Middle Aged, medicine.disease, prostate cancer, Molecular biology, MicroRNAs, medicine.anatomical_structure, click chemistry, biology.protein, Antibody |
| الوصف: | Vesicular miRNA has emerged as a promising marker for various types of cancer, including prostate cancer (PC). In the advanced stage of PC, the cancer-cell-derived small extracellular vesicles (SEVs) may constitute a significant portion of circulating vesicles and may mediate a detectable change in the plasma vesicular miRNA profile. However, SEVs secreted by small tumor in the prostate gland constitute a tiny fraction of circulating vesicles and cause undetectable miRNA pattern changes. Thus, the isolation and miRNA profiling of a specific prostate-derived fraction of SEVs can improve the diagnostic potency of the methods based on vesicular miRNA analysis. Prostate-specific membrane antigen (PSMA) was selected as a marker of prostate-derived SEVs. Super-paramagnetic beads (SPMBs) were functionalized by PSMA-binding DNA aptamer (PSMA–Apt) via a click reaction. The efficacy of SPMB–PSMA–Apt complex formation and PSMA(+)SEVs capture were assayed by flow cytometry. miRNA was isolated from the total population of SEVs and PSMA(+)SEVs of PC patients (n = 55) and healthy donors (n = 30). Four PC-related miRNAs (miR-145, miR-451a, miR-143, and miR-221) were assayed by RT-PCR. The click chemistry allowed fixing DNA aptamers onto the surface of SPMB with an efficacy of up to 89.9%. The developed method more effectively isolates PSMA(+)SEVs than relevant antibody-based technology. The analysis of PC-related miRNA in the fraction of PSMA(+)SEVs was more sensitive and revealed distinct diagnostic potency (AUC: miR-145, 0.76 miR-221, 0.7 miR-451a, 0.65 and miR-141, 0.64) than analysis of the total SEV population. Thus, isolation of prostate-specific SEVs followed by analysis of vesicular miRNA might be a promising PC diagnosis method. |
| وصف الملف: | application/pdf |
| تدمد: | 2073-4409 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ef5d8eded37c73c94624a6eabf457f96 https://pubmed.ncbi.nlm.nih.gov/34572021 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....ef5d8eded37c73c94624a6eabf457f96 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20734409 |
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