Pseudomonas aeruginosa mutants defective in glucose uptake have pleiotropic phenotype and altered virulence in non-mammal infection models
| العنوان: | Pseudomonas aeruginosa mutants defective in glucose uptake have pleiotropic phenotype and altered virulence in non-mammal infection models |
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| المؤلفون: | Matteo Raneri, Irene Bianconi, Palma Ferrante, Giordano Rampioni, Clelia Peano, Chiara Dalmasio, Eva Pinatel, Livia Leoni, Olivier Jousson, Federica Briani |
| المساهمون: | Raneri, Matteo, Pinatel, Eva, Peano, Clelia, Rampioni, Giordano, Leoni, Livia, Bianconi, Irene, Jousson, Olivier, Dalmasio, Chiara, Ferrante, Palma, Briani, Federica |
| المصدر: | Scientific reports (Nature Publishing Group) 8 (2018). doi:10.1038/s41598-018-35087-y info:cnr-pdr/source/autori:Raneri, Matteo; Pinatel, Eva; Peano, Clelia; Rampioni, Giordano; Leonia, Livia; Bianconi, Irene; Jousson, Olivier; Dalmasio, Chiara; Ferrante, Palma; Briani, Federica/titolo:Pseudomonas aeruginosa mutants defective in glucose uptake have pleiotropic phenotype and altered virulence in non-mammal infection models/doi:10.1038%2Fs41598-018-35087-y/rivista:Scientific reports (Nature Publishing Group)/anno:2018/pagina_da:/pagina_a:/intervallo_pagine:/volume:8 Scientific Reports, Vol 8, Iss 1, Pp 1-15 (2018) Scientific Reports |
| بيانات النشر: | Nature Publishing Group, London , Regno Unito, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | 0301 basic medicine, Transcription, Genetic, Glucose uptake, 030106 microbiology, Mutant, RNA-sequencing, lcsh:Medicine, Virulence, Moths, Biology, medicine.disease_cause, Models, Biological, Article, 03 medical and health sciences, glucose uptake, Pseudomonas aeruginosa, virulence, quorum sensing, biofilm, transcriptomics, medicine, Animals, Pseudomonas Infections, Caenorhabditis elegans, lcsh:Science, Gene, Multidisciplinary, Bacteria, Pseudomonas aeruginosa, lcsh:R, Glucose transporter, Quorum Sensing, Genetic Pleiotropy, Gene Expression Regulation, Bacterial, Phenotype, Carbon, Cell biology, Disease Models, Animal, Glucose, Genes, Bacterial, Biofilms, Mutation, Pyocyanine, lcsh:Q, Transcriptome, Bacterial outer membrane, Oligopeptides, Oxidation-Reduction |
| الوصف: | Pseudomonas spp. are endowed with a complex pathway for glucose uptake that relies on multiple transporters. In this work we report the construction and characterization of Pseudomonas aeruginosa single and multiple mutants with unmarked deletions of genes encoding outer membrane (OM) and inner membrane (IM) proteins involved in glucose uptake. We found that a triple ΔgltKGF ΔgntP ΔkguT mutant lacking all known IM transporters (named GUN for Glucose Uptake Null) is unable to grow on glucose as unique carbon source. More than 500 genes controlling both metabolic functions and virulence traits show differential expression in GUN relative to the parental strain. Consistent with transcriptomic data, the GUN mutant displays a pleiotropic phenotype. Notably, the genome-wide transcriptional profile and most phenotypic traits differ between the GUN mutant and the wild type strain irrespective of the presence of glucose, suggesting that the investigated genes may have additional roles besides glucose transport. Finally, mutants carrying single or multiple deletions in the glucose uptake genes showed attenuated virulence relative to the wild type strain in Galleria mellonella, but not in Caenorhabditis elegans infection model, supporting the notion that metabolic functions may deeply impact P. aeruginosa adaptation to specific environments found inside the host. |
| اللغة: | English |
| DOI: | 10.1038/s41598-018-35087-y |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ef16c4eb608da5482b62875317358412 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....ef16c4eb608da5482b62875317358412 |
| قاعدة البيانات: | OpenAIRE |
| DOI: | 10.1038/s41598-018-35087-y |
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