Application of low-intensity pulsed therapeutic ultrasound on mesenchymal precursors does not affect their cell properties
| العنوان: | Application of low-intensity pulsed therapeutic ultrasound on mesenchymal precursors does not affect their cell properties |
|---|---|
| المؤلفون: | Beatriz de Lucas, Aurora Bernal, Laura M. Pérez, Beatriz G. Gálvez |
| المصدر: | ABACUS. Repositorio de Producción Científica Universidad Europea (UEM) Repisalud Instituto de Salud Carlos III (ISCIII) PLoS ONE PLoS ONE, Vol 16, Iss 2, p e0246261 (2021) |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | 0301 basic medicine, Angiogenesis, medicine.medical_treatment, Ultrasonic Therapy, Cell, Gene Expression, Human skin, Biochemistry, Diagnostic Radiology, Mice, 0302 clinical medicine, Cell Movement, Ultrasound Imaging, Interventional Radiology, Medicine and Health Sciences, Medicine, Therapeutic Ultrasound, Tecnología médica, Cytoskeleton, Multidisciplinary, Small nuclear RNA, Radiology and Imaging, Ultrasound, Nucleic acids, Cell Motility, medicine.anatomical_structure, Ultrasonic Waves, Cell Processes, Engineering and Technology, Cellular Structures and Organelles, Research Article, Biotechnology, Célula, Imaging Techniques, Science, Blotting, Western, Células madre mesenquimatosas, Bioengineering, Cell Migration, Research and Analysis Methods, Real-Time Polymerase Chain Reaction, 03 medical and health sciences, Diagnostic Medicine, Genetics, Animals, Humans, Non-coding RNA, Cell Proliferation, Terapia por ultrasonido, Wound Healing, Therapeutic ultrasound, business.industry, Cell growth, Mesenchymal stem cell, Biology and Life Sciences, Mesenchymal Stem Cells, 030206 dentistry, Cell Biology, 030104 developmental biology, Microscopy, Fluorescence, Cancer research, RNA, Medical Devices and Equipment, business, Wound healing, Transcriptome, Developmental Biology |
| الوصف: | Ultrasound is considered a safe and non-invasive tool in regenerative medicine and has been used in the clinic for more than twenty years for applications in bone healing after the approval of the Exogen device, also known as low-intensity pulsed ultrasound (LIPUS). Beyond its effects on bone health, LIPUS has also been investigated for wound healing of soft tissues, with positive results for various cell processes including cell proliferation, migration and angiogenesis. As LIPUS has the potential to treat chronic skin wounds, we sought to evaluate the effects produced by a conventional therapeutic ultrasound device at low intensities (also considered LIPUS) on the migration capacity of mouse and human skin mesenchymal precursors (s-MPs). Cells were stimulated for 3 days (20 minutes per day) using a traditional ultrasound device with the following parameters: 100 mW/cm2 with 20% duty cycle and frequency of 3 MHz. At the parameters used, ultrasound failed to affect s-MP proliferation, with no evident changes in morphology or cell groupings, and no changes at the cytoskeletal level. Further, the migration and invasion ability of s-MPs were unaffected by the ultrasound protocol, and no major changes were detected in the gene/protein expression of ROCK1, integrin β1, laminin β1, type I collagen and transforming growth factor β1. Finally, RNA-seq analysis revealed that only 10 genes were differentially expressed after ultrasound stimulation. Among them, 5 encode for small nuclear RNAs and 2 encode for proteins belonging to the nuclear pore complex. Considering the results overall, while the viability of s-MPs was not affected by ultrasound stimulation and no changes were detected in proliferation/migration, RNA-seq analysis would suggest that s-MPs do respond to ultrasound. The use of 100 mW/cm2 intensity or conventional therapeutic ultrasound devices might not be optimal for the stimulation the properties of cell populations. Future studies should investigate the potential application of ultrasound using variations of the tested parameters. Ministerio de Ciencia e Innovación (SAF 2015-67911-R) 3.752 JCR (2021) Q2, 29/73 Multidisciplinary Sciences 0.852 SJR (2021) Q1, 16/138 Multidisciplinary No data IDR 2021 UEM |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::eefd80ef40243abdf806a620cecc25e2 http://hdl.handle.net/11268/9887 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....eefd80ef40243abdf806a620cecc25e2 |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |