A Preliminary Study Showing the Impact of Genetic and Dietary Factors on GC–MS-Based Plasma Metabolome of Patients with and without PROX1-Genetic Predisposition to T2DM up to 5 Years Prior to Prediabetes Appearance
| العنوان: | A Preliminary Study Showing the Impact of Genetic and Dietary Factors on GC–MS-Based Plasma Metabolome of Patients with and without PROX1-Genetic Predisposition to T2DM up to 5 Years Prior to Prediabetes Appearance |
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| المؤلفون: | Witold Bauer, Adrian Godlewski, Katarzyna Miniewska, Patrycja Mojsak, Fernanda Rey-Stolle, Adam Kretowski, Michal Ciborowski, Coral Barbas, Edyta Adamska-Patruno, Paulina Samczuk |
| المصدر: | Current Issues in Molecular Biology, Vol 43, Iss 39, Pp 513-528 (2021) Current Issues in Molecular Biology Volume 43 Issue 2 Pages 39-528 |
| بيانات النشر: | MDPI AG, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | 0301 basic medicine, Microbiology (medical), NC/HC-meal intake, QH301-705.5, carbohydrates, Physiology, T2DM, 030209 endocrinology & metabolism, Single-nucleotide polymorphism, prediabetes, Biology, Microbiology, fatty acids, 03 medical and health sciences, 0302 clinical medicine, PROX1, Genotype, Metabolome, medicine, Genetic predisposition, GC–MS, Prediabetes, Biology (General), Molecular Biology, plasma, amino acids, Metabolic disorder, Type 2 Diabetes Mellitus, General Medicine, medicine.disease, metabolomics, 030104 developmental biology, Postprandial |
| الوصف: | Risk factors for type 2 diabetes mellitus (T2DM) consist of a combination of an unhealthy, imbalanced diet and genetic factors that may interact with each other. Single nucleotide polymorphism (SNP) in the prospero homeobox 1 (PROX1) gene is a strong genetic susceptibility factor for this metabolic disorder and impaired β-cell function. As the role of this gene in T2DM development remains unclear, novel approaches are needed to advance the understanding of the mechanisms of T2DM development. Therefore, in this study, for the first time, postprandial changes in plasma metabolites were analysed by GC–MS in nondiabetic men with different PROX1 genotypes up to 5 years prior to prediabetes appearance. Eighteen contestants (12 with high risk (HR) and 6 with low risk (LR) genotype) participated in high-carbohydrate (HC) and normo-carbohydrate (NC) meal-challenge tests. Our study concluded that both meal-challenge tests provoked changes in 15 plasma metabolites (amino acids, carbohydrates, fatty acids and others) in HR, but not LR genotype carriers. Postprandial changes in the levels of some of the detected metabolites may be a source of potential specific early disturbances possibly associated with the future development of T2DM. Thus, accurate determination of these metabolites can be important for the early diagnosis of this metabolic disease. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1467-3045 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::eec6af157bc6d6eae5fc5500d7d23c1a https://www.mdpi.com/1467-3045/43/2/39 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....eec6af157bc6d6eae5fc5500d7d23c1a |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14673045 |
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