Choline Acetyltransferase (ChAT) Immunoreactivity in Paraffin Sections of Normal and Diseased Intestines
| العنوان: | Choline Acetyltransferase (ChAT) Immunoreactivity in Paraffin Sections of Normal and Diseased Intestines |
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| المؤلفون: | Derek deSa, Ronald H. Stead, Michael F. Dixon, Elyanne M. Ratcliffe |
| المصدر: | Journal of Histochemistry & Cytochemistry. 46:1223-1231 |
| بيانات النشر: | SAGE Publications, 1998. |
| سنة النشر: | 1998 |
| مصطلحات موضوعية: | 0301 basic medicine, Pathology, medicine.medical_specialty, Histology, Immunocytochemistry, Biology, Choline O-Acetyltransferase, Immunoenzyme Techniques, Rats, Sprague-Dawley, 03 medical and health sciences, chemistry.chemical_compound, Esophagus, mental disorders, medicine, Animals, Humans, Tissue Distribution, Hirschsprung Disease, Intestinal Mucosa, Rats, Wistar, Strongylida Infections, Frozen section procedure, Paraffin Embedding, 030102 biochemistry & molecular biology, Trichinellosis, Acetylcholinesterase, Choline acetyltransferase, Rats, Staining, Ganglion, Intestinal Diseases, 030104 developmental biology, medicine.anatomical_structure, nervous system, chemistry, Rats, Inbred Lew, Cholinergic, Enteric nervous system, Nippostrongylus, Anatomy |
| الوصف: | There is increasing interest in localizing nerves in the intestine, especially specific populations of nerves. At present, the usual histochemical marker for cholinergic nerves in tissue sections is acetylcholinesterase activity. However, such techniques are applicable only to frozen sections and have uncertain specificity. Choline acetyltransferase (ChAT) is also present in cholinergic nerves, and we therefore aimed to establish a paraffin section immunocytochemical technique using an anti-ChAT antibody. Monoclonal anti-choline acetyltransferase (1.B3.9B3) and a biotin-streptavidin detection system were used to study the distribution of ChAT immunoreactivity (ChAT IR) in paraffin-embedded normal and diseased gastrointestinal tracts from both rats and humans. Optimal staining was seen after 6-24 hr of fixation in neutral buffered formalin and overnight incubation in 1 microgram/ml of 1.B3.9B3, with a similar distribution to that seen in frozen sections. In the rat diaphragm (used as a positive control), axons and motor endplates were ChAT IR. Proportions of ganglion cells and nerve fibers in the intramural plexi of both human and rat gastrointestinal tracts were also ChAT IR, as well as extrinsic nerve bundles in aganglionic segments of Hirschsprung's disease. Mucosal cholinergic nerves, however, were not visualized. In addition, non-neuronal cells such as endothelium, epithelium, and inflammatory cells were ChAT IR. We were able to localize ChAT to nerves in formalin-fixed, paraffin-embedded sections. The presence of ChAT IR in non-neuronal cells indicates that this method should be used in conjunction with other antibodies. Nevertheless, it proves to be a useful technique for studying cholinergic neuronal distinction in normal tissues and pathological disorders. |
| تدمد: | 1551-5044 0022-1554 |
| DOI: | 10.1177/002215549804601102 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::eeb7245120ebab861fd80f060591c468 https://doi.org/10.1177/002215549804601102 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....eeb7245120ebab861fd80f060591c468 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15515044 00221554 |
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| DOI: | 10.1177/002215549804601102 |