N-acetyl-L-cysteine (NAC) delays post-ovulatory oocyte aging in mouse

التفاصيل البيبلوغرافية
العنوان: N-acetyl-L-cysteine (NAC) delays post-ovulatory oocyte aging in mouse
المؤلفون: Yue Wang, Yi Hou, Jian Li, Ying-Chun Ouyang, Li Li, Zhen-Bo Wang, Li-Hua Fan, Ying Jing, Qing-Yuan Sun
المصدر: Aging (Albany NY)
بيانات النشر: Impact Journals, 2019.
سنة النشر: 2019
مصطلحات موضوعية: Ovulation, Aging, Antioxidant, medicine.medical_treatment, Intracellular reactive oxygen species, Spindle Apparatus, Mitochondrion, Cytoplasmic Granules, Time-Lapse Imaging, Antioxidants, Andrology, Mice, medicine, Animals, oocyte, Cellular Senescence, Mice, Inbred ICR, Chemistry, ROS, Cell Biology, post-ovulatory aging, Oocyte, Abnormal distribution, In vitro, Acetylcysteine, Mitochondria, medicine.anatomical_structure, Oocytes, Female, Reactive Oxygen Species, N-acetyl-L-cysteine, Intracellular, Research Paper
الوصف: The quality of post-ovulatory oocytes decreases with aging. In this study, we aimed to investigate the effects of N-acetyl-L-cysteine (NAC), a broadly used antioxidant, on oocyte quality in mouse post-ovulatory oocyte aging in vitro. NAC at 0.6mM concentration was added to culture medium (M2), and the quality of oocytes was analyzed at 6h, 12h, 18h and 24h of culture. We found that the frequency of spindle defects decreased in NAC-treated oocytes compared to those without NAC treatment. NAC treatment significantly decreased abnormal distribution of cortical granules (CGs) in oocytes during aging for 18h and 24h. Decreased intracellular reactive oxygen species (ROS) was also observed. Increased intracellular ATP levels and decreased abnormal distribution of mitochondria could be observed with NAC supplementation during post-ovulatory oocyte aging in vitro. These results indicate that NAC will maintain the quality of oocytes, and delay post-ovulatory oocyte aging as studied in the mouse.
اللغة: English
تدمد: 1945-4589
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ee2a94cc6416aaab58f791d6429f86f8
http://europepmc.org/articles/PMC6503888
Rights: OPEN
رقم الانضمام: edsair.doi.dedup.....ee2a94cc6416aaab58f791d6429f86f8
قاعدة البيانات: OpenAIRE